Apolipoprotein A1 Recombinant Rabbit Monoclonal Antibody [PSH21-76]
Catalog# HA724247
Apolipoprotein A1 Recombinant Rabbit Monoclonal Antibody [PSH21-76]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Apolipoprotein A1 Recombinant Rabbit Monoclonal Antibody [PSH21-76]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Apolipoprotein A1 aa 1-267.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
HepG2 cell lysate, human plasma lysates, HepG2, human kidney tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PSH21-76
Reactivity Data
| WB | IF-Cell | IHC-P | FC | |
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| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:1,000
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IHC-P
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1:2,000-1:4,000
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FC
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1:1,000
Target
Function
Apolipoprotein AI (Apo-AI) is a protein that in humans is encoded by the APOA1 gene. As the major component of high-density lipoprotein (HDL) particles, it has a specific role in lipid metabolism. Apolipoprotein AI is the major protein component of high density lipoprotein (HDL) particles in plasma. Chylomicrons secreted from the intestinal enterocyte also contain Apo-AI, but it is quickly transferred to HDL in the bloodstream. The protein, as a component of HDL particles, enables efflux of fat molecules by accepting fats from within cells (including macrophages within the walls of arteries which have become overloaded with ingested fats from oxidized low-density lipoprotein (LDL) particles) for transport (in the water outside cells) elsewhere, including back to LDL particles or to the liver for excretion.
Background References
1. Xu X et al. Apolipoprotein A1-Related Proteins and Reverse Cholesterol Transport in Antiatherosclerosis Therapy: Recent Progress and Future Perspectives. Cardiovasc Ther. 2022 Jan
2. Maarfi F et al. A Study on Multiple Facets of Apolipoprotein A1 Milano. Appl Biochem Biotechnol. 2023 Jul
Subcellular Location
Secreted.
UNIPROT
Synonyms
Apo-AI antibody
ApoA I antibody
ApoA-I antibody
APOA1 antibody
APOA1_HUMAN antibody
Apolipoprotein A-I(1-242) antibody
Apolipoprotein A1 antibody
Apolipoprotein AI antibody
Brp14 antibody
Ltw1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Apolipoprotein A1 on different lysates with Rabbit anti-Apolipoprotein A1 antibody (HA724247) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Huh7 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 26 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724247) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Apolipoprotein A1 on human plasma lysates with Rabbit anti-Apolipoprotein A1 antibody (HA724247) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 26 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724247) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling Apolipoprotein A1 with Rabbit anti-Apolipoprotein A1 antibody (HA724247) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Apolipoprotein A1 antibody (HA724247) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Apolipoprotein A1 antibody (HA724247) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724247) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Apolipoprotein A1 antibody (HA724247) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724247) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HepG2 cells labeling Apolipoprotein A1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA724247, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"