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BAX Mouse Monoclonal Antibody [3-D2]

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Catalog# EM1203

BAX Mouse Monoclonal Antibody [3-D2]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

BAX Mouse Monoclonal Antibody [3-D2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 21 kDa

Positive Control

Daudi cell lysate, Raji cell lysate, HepG2, Hela.

Conjugation

unconjugated

Clone Number

3-D2

RRID

AB_3068739

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgA

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50

Target

Function

BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.

Background References

1. "Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex." Peyerl F.W., Dai S., Murphy G.A., Crawford F., White J., Marrack P., Kappler J.W. Cell Death Differ. 14:447-452(2006)

2. "BAX activation is initiated at a novel interaction site." Gavathiotis E., Suzuki M., Davis M.L., Pitter K., Bird G.H., Katz S.G., Tu H.C., Kim H., Cheng E.H., Tjandra N., Walensky L.D. Nature 455:1076-1081(2007)

3. "Mutation to Bax beyond the BH3 domain disrupts interactions with pro-survival proteins and promotes apoptosis." Czabotar P.E., Lee E.F., Thompson G.V., Wardak A.Z., Fairlie W.D., Colman P.M. J. Biol. Chem. 286:7123-7131(2010)

Sequence Similarity

Belongs to the Bcl-2 family.

Tissue Specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Subcellular Location

Mitochondrion membrane, cytoplasm

UNIPROT

SwissProt: Q07812 Human

SwissProt: Q07813 Mouse

Synonyms

Apoptosis regulator BAX antibody

BAX antibody

Bax-protein antibody

BAX_HUMAN antibody

BAXA antibody

Baxdelta2G9 antibody

Baxdelta2G9omega antibody

Baxdelta2omega antibody

Bcl-2-like protein 4 antibody

BCL2 associated X protein antibody

Expand

Images

  • Western blot analysis of BAX on different lysates with Mouse anti-BAX antibody (EM1203) at 1/500 dilution.<br /><br />Lane 1: Daudi cell lysate<br />Lane 2: Raji cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 2 minutes;<br /><br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1203) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BAX on different lysates with Mouse anti-BAX antibody (EM1203) at 1/500 dilution.

    Lane 1: Daudi cell lysate
    Lane 2: Raji cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 2 minutes;

    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1203) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • ICC staining of BAX in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1203, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of BAX in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1203, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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