BLBP Recombinant Rabbit Monoclonal Antibody [PSH0-89]
Catalog# HA721524
BLBP Recombinant Rabbit Monoclonal Antibody [PSH0-89]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
BLBP Recombinant Rabbit Monoclonal Antibody [PSH0-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human BLBP aa 1-132 / 132.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 15 kDa
Positive Control
Human brain tissue lysate, human cerebellum tissue lysate, human brain tissue, human brain glioma tissue.
Conjugation
unconjugated
Clone Number
PSH0-89
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:3,000-1:5,000
Target
Function
Fatty acid binding protein 7, brain (FABP7; also brain lipid binding protein, BLBP), is a human gene. The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism. FABP7 is expressed, during development, in radial glia by the activation of Notch receptors. Reelin was shown to induce FABP7 expression in neural progenitor cells via Notch-1 activation. According to one study, FABP7 binds DHA with the highest affinity among all of the FABPs.
Background References
1. Sabnis DH et al. BLBP Is Both a Marker for Poor Prognosis and a Potential Therapeutic Target in Paediatric Ependymoma. Cancers (Basel). 2021 Apr
2. Holst CB et al. The HOPX and BLBP landscape and gliogenic regions in developing human brain. J Anat. 2023 Feb 16
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
B FABP antibody
B-FABP antibody
BFABP antibody
BLBP antibody
Brain lipid binding protein antibody
Brain lipid-binding protein antibody
Brain-type fatty acid-binding protein antibody
DKFZp547J2313 antibody
FABP 7 antibody
FABP7 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA721524) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Human cerebellum tissue lysate
Lane 3: Mouse lung tissue lysate (negative)
Lane 4: MCF7 cell lysate (negative)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721524) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-BLBP antibody (HA721524) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721524) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human brain glioma tissue with Rabbit anti-BLBP antibody (HA721524) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721524) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-BLBP antibody (HA721524) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721524) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
BLBP Recombinant Rabbit Monoclonal Antibody [PSH0-88] - BSA and Azide free
Application: WB
Reactivity: Human
Conjugate: unconjugated
BLBP Recombinant Rabbit Monoclonal Antibody [PSH0-89] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
