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BMP2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER80602

BMP2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

BMP2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human BMP2 aa 51-100.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Caco-2 cell lysate, C2C12 cell lysate, Hela, MCF-7, rat small intestine tissue, human breast cancer tissue, mouse small intestine tissue.

Conjugation

unconjugated

RRID

AB_3069591

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

Target

Function

Bone morphogenetic protein 2 or BMP-2 belongs to the TGF-β superfamily of proteins. BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP-2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2 may be involved in white adipogenesis and may have metabolic effects.

Background References

1. Li, K.L., et al. 2012. p53 negatively regulates the osteogenic differentiation of vascular smooth muscle cells in mice with chronic kidney disease. Cardiovasc. J. Afr. 23: e1-e9.

2. Adas, G., et al. 2011. Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study). Langenbecks Arch. Surg. 396: 115-126.

Sequence Similarity

Belongs to the TGF-beta family.

Tissue Specificity

Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P12643 Human

SwissProt: P21274 Mouse

SwissProt: P49001 Rat

Synonyms

BDA2 antibody

BMP-2 antibody

BMP-2A antibody

Bmp2 antibody

BMP2_HUMAN antibody

BMP2A antibody

Bone morphogenetic protein 2 antibody

Bone morphogenetic protein 2A antibody

Images

  • Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (<a href="/products/ER80602" style="font-weight: bold;text-decoration: underline;">ER80602</a>) at 1/1,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate<br />Lane 2: C2C12 cell lysate<br /><br />Predicted band size: 45 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER80602" style="font-weight: bold;text-decoration: underline;">ER80602</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (ER80602) at 1/1,000 dilution.

    Lane 1: Caco-2 cell lysate
    Lane 2: C2C12 cell lysate

    Predicted band size: 45 kDa
    Observed band size: 38 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80602) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

    ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

  • ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

    ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

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BMP2 Recombinant Rabbit Monoclonal Antibody [JE10-29]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Products with the same target and pathway

metafield image

BMP2 Recombinant Rabbit Monoclonal Antibody [JE10-29]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated