BOB1 Rabbit Polyclonal Antibody
Catalog# ER1803-97
BOB1 Rabbit Polyclonal Antibody
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
BOB1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human BOB1 aa 10-180.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
35 kDa (predicted molecular weight: 27 kDa)
Positive Control
Daudi.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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FC
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1:50-1:100
Target
Function
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Reduced activation of Bob1-deficient B cells following antigenic stimulation is often reported. The action of Bob1 on B cell maturation and tolerance could be mediated by effects on B cell activation, in part by regulating the expression of immunosuppressive miRNAs. Bob1 has long been considered a B cell‐specific factor that interacts with the transcription factors Oct1 and Oct2 to enhance octamer‐dependent transcription. Bob1 can directly bind to and transactivate the promoters of Bcl6 and Btla.
Background References
1. Galiegue-Zouitina S et al. Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation. C R Acad Sci III Sci Vie 318:1125-1131 (1995).
2. John M. Lindner et al. A C-Terminal Acidic Domain Regulates Degradation of the Transcriptional Coactivator Bob1. Mol Cell Biol. 33(23):4628-40 (2013).
Sequence Similarity
Belongs to the POU2AF1 family.
Tissue Specificity
B-cell specific.
Post-translational Modification
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Subcellular Location
Nucleus.
Synonyms
B cell Oct binding protein 1 antibody
B cell specific coactivator OBF 1 antibody
B cell specific coactivator OBF1 antibody
B-cell-specific coactivator OBF-1 antibody
BOB 1 antibody
BOB-1 antibody
OBF 1 antibody
OBF1 antibody
OBF1_HUMAN antibody
OCA B antibody
ExpandImages
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Western blot analysis of BOB1 on Daudi lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of BOB1 was done on Daudi cells. The cells were fixed, permeabilized and stained with BOB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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