BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
Catalog# HA601345
BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
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WB
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Human
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Mouse
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Rat
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HA610205
不含抗保成分
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unconjugated
Overview
Product Name
BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human BRG1 aa 230-280.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 185 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
A5C9-R
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP.
Background References
1. Park J.I. et. al. Telomerase modulates Wnt signalling by association with target gene chromatin. Nature 460:66-72(2009).
Subcellular Location
Nucleus.
Synonyms
ATP dependent helicase SMARCA4 antibody
ATP-dependent helicase SMARCA4 antibody
BAF 190 antibody
BAF190 antibody
BAF190A antibody
Brahma protein like 1 antibody
BRG1 antibody
BRG1 associated factor 190A antibody
BRG1 protein antibody
BRG1-associated factor 190A antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of BRG1 on different lysates with Mouse anti-BRG1 antibody (HA601345) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: A549 cell lysate (negative)
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 185 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601345) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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