Beta-Arrestin 1 Rabbit Polyclonal Antibody
Catalog# ER1802-13
Beta-Arrestin 1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Beta-Arrestin 1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within N-terminal human Beta-Arrestin 1.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 47 kDa
Positive Control
Human placenta tissue lysate, A549, HUVEC, LOVO, rat brain tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, Jurkat.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
Target
Function
The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Members of the β-Arrestin family regulate receptor binding to G proteins. β-Arrestins have been found to be located at postsynaptic sites, where they are thought to act in concert with βARK (βARK1, also designated GRK 2, or βARK2, also designated GRK 3) to regulate G protein-coupled neurotransmitter receptors. Expression of β-Arrestin-1 and b-Arrestin-2 is seen predominantly in spleen and neuronal tissues. It has been shown that β-Arrestin-1 expression is modulated by intracellular cAMP, which may be a novel mechanism for the regulation of receptor-mediated responses.
Background References
暂无
Sequence Similarity
Belongs to the arrestin family.
Post-translational Modification
Constitutively phosphorylated at Ser-412 in the cytoplasm. At the plasma membrane, is rapidly dephosphorylated, a process that is required for clathrin binding and ADRB2 endocytosis but not for ADRB2 binding and desensitization. Once internalized, is rephosphorylated.; The ubiquitination status appears to regulate the formation and trafficking of beta-arrestin-GPCR complexes and signaling. Ubiquitination appears to occur GPCR-specific. Ubiquitinated by MDM2; the ubiquitination is required for rapid internalization of ADRB2. Deubiquitinated by USP33; the deubiquitination leads to a dissociation of the beta-arrestin-GPCR complex. Stimulation of a class A GPCR, such as ADRB2, induces transient ubiquitination and subsequently promotes association with USP33.
Subcellular Location
Cytoplasm, Membrane, Nucleus.
Synonyms
ARB1 antibody
ARR1 antibody
ARRB1 antibody
ARRB1_HUMAN antibody
Arrestin 2 antibody
Arrestin beta 1 antibody
Arrestin beta-1 antibody
Beta-arrestin-1 antibody
Images
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Western blot analysis of beta Arrestin 1 on human placenta tissue lysate using anti-beta Arrestin 1 antibody at 1/500 dilution.
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ICC staining beta Arrestin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining beta Arrestin 1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining beta Arrestin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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