BubR1 Recombinant Rabbit Monoclonal Antibody [PSH29-05]
Catalog# HA724833
BubR1 Recombinant Rabbit Monoclonal Antibody [PSH29-05]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
BubR1 Recombinant Rabbit Monoclonal Antibody [PSH29-05]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human BubR1 aa 1-200.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 120 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse testis tissue lysate, Mouse thymus tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
PSH29-05
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:200
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IP
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1-2μg/sample
Target
Function
Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.
Background References
1. Pun R et al. BubR1 and SIRT2: Insights into aneuploidy, aging, and cancer. Semin Cancer Biol. 2024 Nov
2. Pun R et al. BubR1 Insufficiency Drives Transcriptomic Alterations and Pathology Associated With Cardiac Aging and Heart Failure. Aging Cell. 2025 Sep
Subcellular Location
Nucleus, Cytoplasm, Cytoskeleton.
Synonyms
Beta homolg of S. cerevisiae BUB 1 antibody
Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody
BUB 1B antibody
BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody
Bub1A antibody
BUB1B antibody
BUB1B_HUMAN antibody
BUB1beta antibody
BUBR1 antibody
Budding Uninhibited by Benzimidazoles 1 beta antibody
ExpandImages
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Western blot analysis of BubR1 on different lysates with Rabbit anti-BubR1 antibody (HA724833) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 4: Ramos (Human Burkitt's lymphoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724833, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 120 kDa
Observed band size: 120 kDa -
Western blot analysis of BubR1 on different lysates with Rabbit anti-BubR1 antibody (HA724833) at 1/5,000 dilution.
Lane 1: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate (15 µg/Lane)
Lane 2: Mouse testis tissue lysate (20 µg/Lane)
Lane 3: Mouse thymus tissue lysate (20 µg/Lane)
Lane 4: Rat testis tissue lysate (20 µg/Lane)
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724833, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 120 kDa
Observed band size: 120 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724833, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724833, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Thymus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724833, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Thymus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724833, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Immunoprecipitation (IP)
BubR1 was immunoprecipitated in 0.2 mg Raji (Human Burkitt's lymphoma cell) cell lysate with HA724833 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724833 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate (input)
Lane 2: HA724833 IP in Raji cell lysate
Lane 3: Rabbit IgG instead of HA724833 in Raji cell lysate
Exposure time: 3 minutes
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724833, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 120 kDa
Observed band size: 120 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"