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CASK Mouse Monoclonal Antibody [A1C5]

Usd: 350

Specification

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Catalog# EM1902-09

CASK Mouse Monoclonal Antibody [A1C5]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CASK Mouse Monoclonal Antibody [A1C5]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human CASK aa 311-513 / 926.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 105 kDa

Positive Control

SiHa cell lysate, A549 cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human prostate cancer tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

A1C5

RRID

AB_3068953

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000

Target

Function

Peripheral plasma membrane protein CASK is a protein that in humans is encoded by the CASK gene. This gene is also known by several other names: CMG 2 (CAMGUK protein 2), calcium/calmodulin-dependent serine protein kinase 3 and membrane-associated guanylate kinase 2.Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.

Background References

1. Zhang K et al. CASK, APBA1, and STXBP1 collaborate during insulin secretion. Mol Cell Endocrinol. 2021 Jan

2. Qu J et al. CASK regulates Notch pathway and functions as a tumor promoter in pancreatic cancer. Arch Biochem Biophys. 2021 Apr

Sequence Similarity

In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.; Belongs to the MAGUK family.

Tissue Specificity

Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.

Subcellular Location

Nucleus, Cytoplasm, Cell membrane.

UNIPROT

SwissProt: O14936 Human

SwissProt: O70589 Mouse

SwissProt: Q62915 Rat

Synonyms

CAGH39 antibody

Caki antibody

Calcium/calmodulin dependent serine protein kinase antibody

Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody

Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody

Calcium/calmodulin-dependent serine protein kinase antibody

CAMGUK antibody

CAMGUK protein antibody

CAMGUK, drosophila, homolog of antibody

casK antibody

Expand

Images

  • Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution.<br /><br />Lane 1: SiHa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: U-87 MG cell lysate<br />Lane 4: Neuro-2a cell lysate<br />Lane 5: Human brain tissue lysate<br />Lane 6: Mouse brain tissue lysate<br />Lane 7: Rat brain tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 105 kDa<br />Observed band size: 105 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution.

    Lane 1: SiHa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: U-87 MG cell lysate
    Lane 4: Neuro-2a cell lysate
    Lane 5: Human brain tissue lysate
    Lane 6: Mouse brain tissue lysate
    Lane 7: Rat brain tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 105 kDa
    Observed band size: 105 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD CASK cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 105 kDa<br />Observed band size: 105 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD CASK cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 105 kDa
    Observed band size: 105 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CASK antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-09) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-CASK antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-09) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-CASK antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1902-09" style="font-weight: bold;text-decoration: underline;">EM1902-09</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-09) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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