CCL5 Recombinant Rabbit Monoclonal Antibody [PSH24-74] - BSA and Azide free
Catalog# HA751952
CCL5 Recombinant Rabbit Monoclonal Antibody [PSH24-74] - BSA and Azide free
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WB
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IF-Cell
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FC
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Human
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HA724471
含抗保成分
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unconjugated
Overview
Product Name
CCL5 Recombinant Rabbit Monoclonal Antibody [PSH24-74] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 10 kDa
Positive Control
NK-92 (Human natural killer cells) cell lysate.
Conjugation
unconjugated
Clone Number
PSH24-74
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:500
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FC
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1:1,000
Target
Function
Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. The gene has been discovered in 1990 by in situ hybridisation and it is localised on 17q11.2-q12 chromosome. It is also known as RANTES (regulated on activation, normal T-cell expressed and secreted). CCL5 belongs to the CC subfamily of chemokines, due to its adjacent cysteines near N terminus. It is an 8kDa protein acting as a classical chemotactic cytokine or chemokine. It consists of 68 amino acids. CCL5 is proinflammatory chemokine, recruiting leukocytes to the site of inflammation. It is chemotactic for T cells, eosinophils, and basophils, but also for monocytes, natural-killer (NK) cells, dendritic cells and mastocytes. With the help of particular cytokines (i.e., IL-2 and IFN-γ) that are released by T cells, CCL5 also induces the proliferation and activation of certain NK cells to form CHAK (CC-Chemokine-activated killer) cells. It is also an HIV-suppressive factor released from CD8+ T cells
Background References
1. Zeng Z et al. CCL5/CCR5 axis in human diseases and related treatments. Genes Dis. 2022 Jan
2. Chen K et al. Single cell RNA-seq reveals the CCL5/SDC1 receptor-ligand interaction between T cells and tumor cells in pancreatic cancer. Cancer Lett. 2022 Oct
Subcellular Location
Secreted.
UNIPROT
Synonyms
Beta chemokine RANTES antibody
Beta chemokine RANTES precursor antibody
C C motif chemokine 5 antibody
CCL 5 antibody
CCL5 antibody
CCL5_HUMAN antibody
Chemokine (C C motif) ligand 5 antibody
Chemokine CC Motif Ligand 5 antibody
D17S136E antibody
EoCP antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CCL5 on different lysates with Rabbit anti-CCL5 antibody (HA751952) at 1/5,000 dilution.
Lane 1: NK-92 (Human natural killer cells) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 59 seconds; ECL: K1802
Jurkat is a negative control (PMID: 15239133).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751952, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 10 kDa
Observed band size: 10 kDa -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: NK-92 (Human natural killer cells)/Jurkat (Human T-lymphoblastic cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA751952, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Negative control: Jurkat (PMID:15239133). -
☑ Relative expression (RE)
Application: Flow Cytometry (Intra)
Species: Human
Sample: NK-92 (Human natural killer cells)/Jurkat (Human T-lymphoblastic cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA751952 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: Jurkat (PMID: 15239133).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"