CD10 Mouse Monoclonal Antibody [A1G4]

Western blot analysis of CD10 on different lysates with Mouse anti-CD10 antibody (EM1901-25) at 1/5,000 dilution.

Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate
Lane 2: Ramos (Human Burkitt's lymphoma cell) cell lysate
Lane 3: Daudi (Human Burkitt's lymphoma cell) cell lysate
Lane 4: HT-29 (Human colorectal adenocarcinoma cell) cell lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Mouse lung tissue lysate
Lane 7: Rat kidney tissue lysate
Lane 8: Rat lung tissue lysate

Cell lysates at 15 µg/Lane;tissue lysates at 30 µg/Lane.
Exposure time: 4 seconds; ECL: K1801

HT-29 is a negative control (PMID:15286660).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1901-25, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 86 kDa
Observed band size: 100 kDa

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-25, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD10 antibody (EM1901-25) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-25) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-25, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of CD10 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-25, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD10 antibody (EM1901-25) at 1/800 dilution.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with EM1901-25 for 30 mins at room temperature. The immunostaining was performed on a Dartmon AS330 PLUS instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.