CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R] - BSA and Azide free

☑ Relative expression (RE)

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA610009) at 1/1,000 dilution.

Lane 1: Caco-2 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: HeLa cell lysate (negative)
Lane 4: NCCIT cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 97 kDa
Observed band size: 120 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610009) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (HA610009) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA610009) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.