CD169 Recombinant Rabbit Monoclonal Antibody [PSH05-21] - BSA and Azide free
Catalog# HA750992
CD169 Recombinant Rabbit Monoclonal Antibody [PSH05-21] - BSA and Azide free
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WB
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
CD169 Recombinant Rabbit Monoclonal Antibody [PSH05-21] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CD169 aa 1-1,000 / 1,709.
Species Reactivity
Human, Mouse
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 183 kDa
Positive Control
K-562 cell lysate, RAW264.7 cell lysate, THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours, RAW264.7 cells treated with 1μg/mL LPS for 24 hours.
Conjugation
unconjugated
Clone Number
PSH05-21
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Sialoadhesin is a cell adhesion molecule found on the surface of macrophages. It is found in especially high amounts on macrophages of the spleen, liver, lymph node, bone marrow, colon, and lungs. Also, in patients with rheumatoid arthritis, the protein has been found in great amounts on macrophages of the affected tissues. It is defined as an I-type lectin, since it contains 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains), and thus also belongs to the immunoglobulin superfamily (IgSF). Sialoadhesin binds to certain molecules called sialic acids. During this binding process a salt bridge (protein) is formed between a highly conserved arginine residue (from the v-set domain to the 3'-sialyllactose) and the carboxylate group of the sialic acid. Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. Alternate names for sialoadhesin include siglec-1 and CD169 (cluster of differentiation 169). Sialoadhesin predominantly binds neutrophils, but can also bind monocytes, natural killer cells, B cells and a subset of cytotoxic T cells by interacting with sialic acid molecules in the ligands on their surfaces. Sialoadhesin (CD169) positive macrophages, along with mesenchymal stem cells and beta-adrenergic neurons, form the hematopoietic stem cell niche in the bone marrow. CD169+ macrophages mediate signaling between the various cells and seem to promote hematopoietic stem cell retention to the niche.
Background References
1. Kim HJ et al. Blood monocyte-derived CD169(+) macrophages contribute to antitumor immunity against glioblastoma. Nat Commun. 2022 Oct
2. Yeung ST et al. CD169+ macrophage intrinsic IL-10 production regulates immune homeostasis during sepsis. Cell Rep. 2023 Mar
Subcellular Location
Cell membrane, Secreted.
Synonyms
CD169 antibody
CD169 antigen antibody
dJ1009E24.1 antibody
DKFZp667F058 antibody
FLJ00051 antibody
FLJ00055 antibody
FLJ00073 antibody
FLJ00411 antibody
FLJ32150 antibody
Sialic acid binding Ig like lectin 1 antibody
ExpandImages
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This data was developed using HA722246, the same antibody clone in a different buffer formulation.
Western blot analysis of CD169 on different lysates with Rabbit anti-CD169 antibody (HA722246) at 1/2,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 183 kDa
Observed band size: 220 kDa
Exposure time: 28 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
This data was developed using HA722246, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of THP-1 cells (left) and THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours (right) labeling CD169.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722246, 0.5μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
☑ Cell treatment (CT)
This data was developed using HA722246, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of RAW264.7 cells (left) and RAW264.7 cells treated with 1μg/mL LPS for 24 hours (right) labeling CD169.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722246, 0.5μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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