CD1a Mouse Monoclonal Antibody [A3F9]
Catalog# EM1901-89
CD1a Mouse Monoclonal Antibody [A3F9]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
CD1a Mouse Monoclonal Antibody [A3F9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CD1a aa 50-250.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 37 kDa
Positive Control
K562 cell lysates, human skin tissue.
Conjugation
unconjugated
Clone Number
A3F9
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:1,000
Target
Function
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants.
Background References
1. Maciocia PM. et. al. Anti-CD1a CAR T cells to selectively target T-ALL. Blood. 2019 May.
2. Ferrufino-Schmidt MC. et. al. Is CD1a useful for leishmaniasis diagnosis in the New World? J Cutan Pathol. 2019 Jan.
Tissue Specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Subcellular Location
Cell membrane, endosome membrane, membrane raft.
UNIPROT
Synonyms
CD 1a antibody
CD1 antibody
CD1a antibody
CD1A Antigen antibody
CD1A antigen, a polypeptide antibody
CD1a molecule antibody
CD1A_HUMAN antibody
cluster of differentiation 1 A antibody
cortical thymocyte antigen CD1A antibody
differentiation antigen CD1 alpha 3 antibody
ExpandImages
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Western blot analysis of CD1a on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-CD1a antibody (EM1901-89) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-89) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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