Skip to content

CD3 epsilon Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# ER80501

CD3 epsilon Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD3 epsilon Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within C-terminal human CD3 epsilon.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 23 kDa

Positive Control

Jurkat cell lysate, Mouse spleen tissue lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate.

Conjugation

unconjugated

RRID

AB_3069589

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) These chains associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes.

Background References

1. Weiss A. Signal transduction. Zapping tandem SH2 domains. Nature 377: 17-18 (1995).

2. Neumeister E N et al. Binding of ZAP-70 to phosphorylated T-cell receptor ζ and ε enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins. Mol Cell Biol 15:3171-3178 (1995).

Post-translational Modification

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P07766 Human

SwissProt: P22646 Mouse

Entrez Gene: 315609 Rat

Synonyms

CD3 epsilon antibody

CD3e antibody

CD3e antigen epsilon polypeptide (TiT3 complex) antibody

CD3E antigen epsilon polypeptide antibody

CD3E antigen, epsilon subunit antibody

CD3e molecule epsilon antibody

CD3e molecule, epsilon (CD3 TCR complex) antibody

CD3e molecule, epsilon (CD3-TCR complex) antibody

CD3E_HUMAN antibody

IMD18 antibody

Expand

Images

  • Western blot analysis of CD3 epsilon on different lysates with Rabbit anti-CD3 epsilon antibody (<a href="/products/ER80501" style="font-weight: bold;text-decoration: underline;">ER80501</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (20 µg/Lane)<br />Lane 2: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 3: Mouse thymus tissue lysate (40 µg/Lane)<br />Lane 4: Rat thymus tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 23 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER80501" style="font-weight: bold;text-decoration: underline;">ER80501</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD3 epsilon on different lysates with Rabbit anti-CD3 epsilon antibody (ER80501) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate (20 µg/Lane)
    Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 3: Mouse thymus tissue lysate (40 µg/Lane)
    Lane 4: Rat thymus tissue lysate (40 µg/Lane)

    Predicted band size: 23 kDa
    Observed band size: 20 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

Products with the same target and pathway

metafield image

CD3 epsilon Recombinant Rabbit Monoclonal Antibody [SY0239] - BSA and Azide free

Application: WB,IHC-P,IF-Cell,IF-Tissue,FC,IP

Reactivity: Human

Conjugate: unconjugated

metafield image

CD3 epsilon Recombinant Rabbit Monoclonal Antibody [SY0239]

Application: WB,IHC-P,IF-Cell,IF-Tissue,FC,IP

Reactivity: Human

Conjugate: unconjugated

metafield image

CD3 epsilon Recombinant Rabbit Monoclonal Antibody [JE17-74]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated