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CD44 Recombinant Rabbit Monoclonal Antibody [ST57-03]

Usd: 385

Specification

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Catalog# ET1609-74

CD44 Recombinant Rabbit Monoclonal Antibody [ST57-03]

Application

  • WB

  • IHC-P

  • mIHC

  • IF-Tissue

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD44 Recombinant Rabbit Monoclonal Antibody [ST57-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CD44 aa 150-190.

Species Reactivity

Human

Validated Applications

WB, IHC-P, mIHC, IF-Tissue

Molecular Weight

Predicted band size: 82 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, MDA-MB-231 cell lysate, human breast cancer tissue, human colon cancer tissue, human spleen tissue, human skin tissue.

Conjugation

unconjugated

Clone Number

ST57-03

RRID

AB_3069883

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • mIHC

  • 1:200

  • IF-Tissue

  • 1:100

Target

Function

Cell adhesion molecules (CAMs) are a family of closely related, cell surface glycoproteins that are involved in cell-cell interactions and are thought to play an important role in embryogenesis and development. HCAM, also known as CD44, LHR, MDU2, MDU3, MIC4, Pgp1, HCELL, MUTCH-I or ECMR-III, is a 742 amino acid single-pass type I membrane protein that is involved in hematopoiesis, lymphocyte activation and tumor metastasis. Functioning as a receptor for hyaluronic acid (HA) and interacting with ligands such as osteopontin (OPN), HCAM mediates both cell-cell and cell-matrix interactions, thereby playing an essential role in cell adhesion and cell migration. HCAM contains one Link domain and, due to alternative splicing events, is expressed as multiple isoforms, some of which are designated CD44R, CDw44, CD44S, CD44H (hematopoietic) and CD44E (epithelial). While most of the HCAM splice varients are expressed in tissues throughout the body, one specific isoform, namely CD44H, is expressed at high levels in cancer tissue, suggesting an important role for the CD44H splice varient in tumor progression.

Background References

1. Chen Z et al. Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition. BMC Cancer 15:811 (2015).

2. Collet B et al. Proteomic analysis underlines the usefulness of both primary adherent and stem-like cell lines for studying proteins involved in human glioblastoma. J Proteomics 110C:7-19 (2014).

Tissue Specificity

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

Post-translational Modification

Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.; N-glycosylated.; O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.; Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P16070 Human

Synonyms

LHR antibody

BA-1 antibody

CD 44 antibody

CD44 antibody

CD44 antigen antibody

CD44 molecule (Indian blood group) antibody

CD44 molecule antibody

CD44_HUMAN antibody

CDw44 antibody

Cell surface glycoprotein CD44 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD44 on different lysates with Rabbit anti-CD44 antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: MCF7 cell lysate (negative)<br />Lane 3: A549 cell lysate<br />Lane 4: Jurkat cell lysate (negative)<br />Lane 5: MDA-MB-231 cell lysate<br />Lane 6: LNCaP cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 82 kDa<br />Observed band size: 82 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD44 on different lysates with Rabbit anti-CD44 antibody (ET1609-74) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: MCF7 cell lysate (negative)
    Lane 3: A549 cell lysate
    Lane 4: Jurkat cell lysate (negative)
    Lane 5: MDA-MB-231 cell lysate
    Lane 6: LNCaP cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 82 kDa
    Observed band size: 82 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-74) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CD44 antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CD44 antibody (ET1609-74) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CD44 antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CD44 antibody (ET1609-74) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD44 antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD44 antibody (ET1609-74) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD44 antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD44 antibody (ET1609-74) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CD44 antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-74" style="font-weight: bold;text-decoration: underline;">ET1609-74</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CD44 antibody (ET1609-74) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/100

    Application: IF-Tissue

    Species: Human

    Site: spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/100

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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