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CD45 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1901-29

CD45 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD45 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within C-terminal human CD45.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC, IF-Tissue

Target Molecular Weight

Predicted band size: 147 kDa

Positive Control

Jurkat cell lysates, human tonsil tissue, human spleen tissue, Jurkat.

Conjugation

unconjugated

RRID

AB_3069322

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:500

  • FC

  • 1:50-1:100

  • IF-Tissue

  • 1:100

Target

Function

Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). The protein product of this gene, best known as CD45, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment, and two tandem intracytoplasmic catalytic domains, and thus belongs to the receptor type PTP family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.

Background References

1. Wu L. et. al. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. Mol. Cell. Biol. 22:2673-2686(2002).

2. Gabaev I. et. al. The human cytomegalovirus UL11 protein interacts with the receptor tyrosine phosphatase CD45, resulting in functional paralysis of T cells. PLoS Pathog. 7:E1002432-E1002432(2011).

Sequence Similarity

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

Tissue Specificity

Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.

Post-translational Modification

Heavily N- and O-glycosylated.

Subcellular Location

Cell membrane, Membrane raft.

UNIPROT

SwissProt: P08575 Human

Synonyms

B220 antibody

CD 45 antibody

CD45 antibody

CD45 antigen antibody

CD45R antibody

GP180 antibody

L-CA antibody

LCA antibody

Leukocyte common antigen antibody

loc antibody

Expand

Images

  • Western blot analysis of CD45 on Jurkat (Human T-lymphoblastic cells) lysates with Rabbit anti-CD45 antibody (<a href="/products/ER1901-29" style="font-weight: bold;text-decoration: underline;">ER1901-29</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 1 minutes 50 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1901-29" style="font-weight: bold;text-decoration: underline;">ER1901-29</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 147 kDa<br />Observed band size: 200 kDa

    Western blot analysis of CD45 on Jurkat (Human T-lymphoblastic cells) lysates with Rabbit anti-CD45 antibody (ER1901-29) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minutes 50 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1901-29, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 147 kDa
    Observed band size: 200 kDa

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1901-29" style="font-weight: bold;text-decoration: underline;">ER1901-29</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1901-29" style="font-weight: bold;text-decoration: underline;">ER1901-29</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of CD45 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER1901-29" style="font-weight: bold;text-decoration: underline;">ER1901-29</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of CD45 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-29, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/100

    Application: IF-Tissue

    Species: Human

    Site: spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/100

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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