CD47 Mouse Monoclonal Antibody [1A9]
Catalog# EM1701-38
CD47 Mouse Monoclonal Antibody [1A9]
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD47 Mouse Monoclonal Antibody [1A9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CD47 aa 19-141 (Extracellular).
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Human ovarian carcinoma tissue, human prostate tissue, SH-SY5Y.
Conjugation
unconjugated
Clone Number
1A9
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgM
Purification Method
Protein G affinity purified.
Application Dilution
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IF-Cell
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1:50-1:100
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IHC-P
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1:200-1,000
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FC
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1:50-1:100
Target
Function
CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin (SIRP). Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. CD47 is absent from Rh-null erythrocytes, but does play a role in cell adhesion in non-erythroid cells and may prevent premature elimination of erythrocytes. It may also be involved in membrane permeability changes following viral infection. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. CD47 is expressed on hemopoietic cells, epithelial cells, endothelial cells and fibroblasts and is strongly expressed in brain and mesenchymal cells.
Background References
1. Li DD et al. Late-stage inhibition of autophagy enhances calreticulin surface exposure. Oncotarget 7:80842-80854 (2016).
2. Malek MH et al. Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks of endurance exercise in mice: discontinuous versus continuous training. Exp Physiol 98:807-18 (2013).
Tissue Specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Subcellular Location
Cell membrane, Multi-pass membrane protein.
UNIPROT
Synonyms
Antigen identified by monoclonal antibody 1D8 antibody
Antigenic surface determinant protein OA3 antibody
CD 47 antibody
Cd47 antibody
CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
CD47 antigen antibody
CD47 glycoprotein antibody
CD47 molecule antibody
CD47_HUMAN antibody
IAP antibody
ExpandImages
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ICC staining CD47 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Mouse anti-CD47 antibody (EM1701-38) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-38) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-CD47 antibody (EM1701-38) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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