CD64 Mouse Monoclonal Antibody [A7E4]
Catalog# HA601003
CD64 Mouse Monoclonal Antibody [A7E4]
-
WB
-
Human
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unconjugated
Overview
Product Name
CD64 Mouse Monoclonal Antibody [A7E4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CD64 aa 1-200 / 374.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 43 kDa
Positive Control
THP-1 cell lysates, U937 cell lysates.
Conjugation
unconjugated
Clone Number
A7E4
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
Target
Function
CD64 (Cluster of Differentiation 64) is a type of integral membrane glycoprotein known as an Fc receptor that binds monomeric IgG-type antibodies with high affinity. It is more commonly known as Fc-gamma receptor 1 (FcγRI). After binding IgG, CD64 interacts with an accessory chain known as the common γ chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellular activation. Structurally CD64 is composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail.CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. There are three distinct (but highly similar) genes in humans for CD64 called FcγRIA (CD64A), FcγRIB (CD64B), and FcγRIC (CD64C) that are located on chromosome 1. These three genes produce six different mRNA transcripts; two from CD64A, three from CD64B, and one from CD64C; by alternate splicing of the genes.
Background References
1. Patnaik R. et. al. Neutrophil CD64 a Diagnostic and Prognostic Marker of Sepsis in Adult Critically Ill Patients: A Brief Review. Indian J Crit Care Med. 2020 Dec
2. Walcheck B. et. al. iNK-CD64/16A cells: a promising approach for ADCC? Expert Opin Biol Ther. 2019 Dec
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 64 antibody
CD64 antibody
CD64 antigen antibody
CD64A antibody
CD64b antibody
CD64c antibody
Fc fragment of IgG high affinity Ia receptor antibody
Fc fragment of IgG high affinity Ib receptor antibody
Fc fragment of IgG high affinity Ic receptor antibody
Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody
ExpandImages
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Western blot analysis of CD64 on THP-1 cell lysates with Mouse anti-CD64 antibody (HA601003) at 1/2,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 70 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601003) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD64 on U937 cell lysates with Mouse anti-CD64 antibody (HA601003) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 70 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601003) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Identification and validation of an endotoxin tolerance–based prognostic model with therapeutic insights in sepsis
Journal: Frontiers In Immunology
DOI: 10.3389/fimmu.2025.1707490
IF: 5.9
Application: WB
Reactivity: Human
Publish date: 2026 Jan
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