CD66b Rabbit Polyclonal Antibody
Catalog# IRS127RB
CD66b Rabbit Polyclonal Antibody
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mIHC
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Human
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unconjugated
Overview
Product Name
CD66b Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CD66b aa 1-250.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 38 kDa
Positive Control
Human tonsil tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
Carcinoembryonic antigen-related cell adhesion molecule 8, also designated CD67, CD66b or nonspecific cross-reacting antigen (NCA-95), belongs to the human carcinoembryonic antigen (CEA) family. The CD67 antigen is encoded by the CEACAM8 (CGM6) gene, which is exclusively expressed in neutrophils and eosinophils. In neutrophils, the CEACAM8 gene is primarily detected in the secondary granules within the cytoplasm, but it can also be found in lower amounts on the plasma membrane. The amount of CD67 on the plasma membrane is up-regulated upon granulocyte activation. CD67 has been located on the surface of neutrophilic and eosinophilic granulocytes at late stages of differentiation. It exhibits heterophilic cell adhesion properties with CD66c, which is coexpressed with CD67 in granulocytes. CD67, which is attached to the membrane by a GPI-anchor, is expressed in leukocytes of chronic myeloid leukemia patients and bone marrow and in granulocytes in the spleen, thymus and lungs.
Background References
1. Posabella A. et. al. High density of CD66b in primary high-grade ovarian cancer independently predicts response to chemotherapy. J Cancer Res Clin Oncol. 2020 Jan
2. Huang X. et. al. Prognostic significance of the infiltration of CD163(+) macrophages combined with CD66b(+) neutrophils in gastric cancer. Cancer Med. 2018 May
Subcellular Location
Cell membrane, Cell surface.
UNIPROT
Synonyms
Carcinoembryonic antigen CGM6 antibody
Carcinoembryonic antigen gene family member 6 antibody
Carcinoembryonic antigen related cell adhesion molecule 8 antibody
Carcinoembryonic antigen-related cell adhesion molecule 8 antibody
CD 66b antibody
CD 67 antibody
CD66b antibody
CD66b antigen antibody
CD67 antibody
CD67 antigen antibody
ExpandImages
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD66b antibody (IRS127RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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