CD8 alpha Rabbit Polyclonal Antibody
Catalog# ER2001-19
CD8 alpha Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CD8 alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CD8A aa 1-235.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
MOLT-4 cell lysate, THP-1 cell lysate, mouse thymus tissue lysate, mouse spleen tissue lysate, rat spleen tissue lysate, rat thymus tissue lysate, MCF-7, human spleen tissue, Jurkat.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Background References
1. Addison E.G. et. al. Ligation of CD8alpha on human natural killer cells prevents activation-induced apoptosis and enhances cytolytic activity. Immunology 116:354-361(2005).
Subcellular Location
Cell membrane, Membrane, Secreted.
Synonyms
CD8 antibody
CD8 antigen, alpha polypeptide (p32) antibody
CD8a antibody
CD8a antigen antibody
CD8a molecule antibody
CD8A_HUMAN antibody
Leu2 antibody
Leu2 T lymphocyte antigen antibody
MAL antibody
OKT8 T cell antigen antibody
ExpandImages
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Western blot analysis of CD8 alpha on different lysates with Rabbit anti-CD8 alpha antibody (ER2001-19) at 1/5,000 dilution.
Lane 1: MOLT-4 cell lysate
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: Mouse thymus tissue lysate (40 µg/Lane)
Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
Lane 5: Rat spleen tissue lysate (40 µg/Lane)
Lane 6: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 30 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-19) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of CD8 alpha in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-19, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of CD8 alpha was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-19, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD8 alpha Recombinant Rabbit Monoclonal Antibody [SI18-01]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,mIHC
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
CD8 alpha Recombinant Rabbit Monoclonal Antibody [SI18-01]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,mIHC
Reactivity: Human
Conjugate: unconjugated
