CD99 Recombinant Rabbit Monoclonal Antibody [PD00-13]
Catalog# HA721150
CD99 Recombinant Rabbit Monoclonal Antibody [PD00-13]
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD99 Recombinant Rabbit Monoclonal Antibody [PD00-13]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CD99 aa 148-185 (Cytoplasmic).
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 19 kDa
Positive Control
Human esophagus tissue, human stomach carcinoma tissue, human ovary granulosa tumor tissue.
Conjugation
unconjugated
Clone Number
PD00-13
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000-1:2,000
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IF-Tissue
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1:1,000
Target
Function
CD99 is a transmembrane glycoprotein, also known as MIC2, involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. It is involved in T-cell adhesion, leukocyte migration and differentiation of primitive neuroectodermal cell. CD99 labels lymphocyte, ovarian granulosa cells, pancreatic islet cells, Sertoli cells, CNS ependymal cells and endothelial cells. CD99 has been useful in diagnosis of Ewing's sarcoma, sex cord-stromal tumor, endocrine tumor of pancreas. Additionally, it is found in a subset of other tumors including lymphoblastic lymphoma, breast carcinoma and other malignancies.
Background References
1. Ali A. et. al. CD99 in malignant hematopoiesis. Exp Hematol. 2022 Feb
2. Mannion AJ. et. al. Tumour cell CD99 regulates transendothelial migration via CDC42 and actin remodelling. J Cell Sci. 2021 Aug
Subcellular Location
Membrane.
UNIPROT
Synonyms
12E7 antibody
Antigen identified by monoclonal 12E7, Y homolog antibody
Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
CD99 antibody
CD99 antigen antibody
CD99 molecule antibody
CD99_HUMAN antibody
Cell surface antigen 12E7 antibody
Cell surface antigen HBA 71 antibody
Cell surface antigen O13 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CD99 antibody (HA721150) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721150) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-CD99 antibody (HA721150) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721150) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human ovary granulosa tumor tissue with Rabbit anti-CD99 antibody (HA721150) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721150) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: esophagus
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Primary antibody dilution: 1/500
Antigen retrieval: ER2
Platform: Leica Biosystems BOND® RX
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD99 Recombinant Rabbit Monoclonal Antibody [JF0991]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human
Conjugate: unconjugated
