CDC42 Recombinant Rabbit Monoclonal Antibody [JJ086-04] - BSA and Azide free
Catalog# HA750320
CDC42 Recombinant Rabbit Monoclonal Antibody [JJ086-04] - BSA and Azide free
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WB
-
IF-Cell
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FC
-
IF-Tissue
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Human
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Mouse
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Rat
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ET1701-7
含抗保成分
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unconjugated
Overview
Product Name
CDC42 Recombinant Rabbit Monoclonal Antibody [JJ086-04] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CDC42 aa 30-191 / 191.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC, IF-Tissue
Molecular Weight
Predicted band size: 21 kDa
Positive Control
HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HepG2, NIH/3T3, C6.
Conjugation
unconjugated
Clone Number
JJ086-04
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
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1:500
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FC
-
1:1,000
-
IF-Tissue
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1:500
Target
Function
The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42.
Background References
1. Gerasimcik N et al. The Rho GTPase Cdc42 Is Essential for the Activation and Function of Mature B Cells. J Immunol 194:4750-8 (2015).
2. Francis MK et al. Endocytic membrane turnover at the leading edge is driven by a transient interaction between Cdc42 and GRAF1. J Cell Sci 128:4183-95 (2015).
Sequence Similarity
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
Post-translational Modification
(Microbial infection) AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.; Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI.; (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of CDC42 and leads to actin disassembly.
Subcellular Location
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.
Synonyms
CDC42 antibody
CDC42_HUMAN antibody
CDC42Hs antibody
Cell division control protein 42 homolog antibody
Cell division cycle 42 (GTP binding protein 25kDa) antibody
Cell division cycle 42 antibody
dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
G25K antibody
G25K GTP-binding protein antibody
ExpandImages
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Western blot analysis of CDC42 on different lysates with Rabbit anti-CDC42 antibody (HA750320) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: C6 cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750320) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling CDC42 with Rabbit anti-CDC42 antibody (HA750320) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC42 antibody (HA750320) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling CDC42 with Rabbit anti-CDC42 antibody (HA750320) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC42 antibody (HA750320) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling CDC42 with Rabbit anti-CDC42 antibody (HA750320) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC42 antibody (HA750320) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HepG2 cells labeling CDC42.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750320, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling CDC42.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750320, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of C6 cells labeling CDC42.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750320, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Application: IF-Tissue
Species: Mouse
Site: colon
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Application: IF-Tissue
Species: Rat
Site: colon
Sample: Paraffin-embedded section
Antibody concentration: 1/500
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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