Cdk2 Mouse Monoclonal Antibody [10-D7]

Function

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1.

Background References

1. "Human Speedy: a novel cell cycle regulator that enhances proliferation through activation of Cdk2." Porter L.A., Dellinger R.W., Tynan J.A., Barnes E.A., Kong M., Lenormand J.-L., Donoghue D.J. J. Cell Biol. 157:357-366(2002)

2. "Cyclin A/cdk2 coordinates centrosomal and nuclear mitotic events." De Boer L., Oakes V., Beamish H., Giles N., Stevens F., Somodevilla-Torres M., Desouza C., Gabrielli B. Oncogene 27:4261-4268(2008)

Sequence Similarity

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Post-translational Modification

Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.; Nitrosylated after treatment with nitric oxide (DETA-NO).

Subcellular Location

Cytoplasm, nucleus

UNIPROT

SwissProt: P24941 Human

Synonyms