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CDKN1A/P21 Rabbit Polyclonal Antibody

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Catalog# ER1906-07

CDKN1A/P21 Rabbit Polyclonal Antibody

Application

  • IHC-P

  • IF-Cell

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CDKN1A/P21 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from human P21 21-100/164

Species Reactivity

Human, Rat

Validated Applications

IHC-P, IF-Cell, IF-Tissue, FC

Molecular Weight

18 KDa

Positive Control

Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:100-500

  • IF-cell

  • 1:100

  • IF-tissue

  • 1:100-500

  • FC

  • 1μg/Test: 1:5000-10000

Target

Function

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Background References

暂无

Subcellular Location

Cytoplasmic and Nuclear.

UNIPROT

SwissProt: P38936 Human

Synonyms

CAP20 antibody

CDK-interacting protein 1 antibody

CDKI antibody

CDKN1 antibody

Cdkn1a antibody

CDN1A_HUMAN antibody

CIP1 antibody

Cyclin Dependent Kinase Inhibitor 1A antibody

Cyclin-dependent kinase inhibitor 1 antibody

Cyclin-dependent kinase inhibitor 1A (P21) antibody

Expand

Images

  • Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P21) Polyclonal Antibody, Unconjugated (<a href="/products/ER1906-07" style="font-weight: bold;text-decoration: underline;">ER1906-07</a>) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P21) Polyclonal Antibody, Unconjugated (ER1906-07) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  • Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(<a href="/products/ER1906-07" style="font-weight: bold;text-decoration: underline;">ER1906-07</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(ER1906-07) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(<a href="/products/ER1906-07" style="font-weight: bold;text-decoration: underline;">ER1906-07</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(ER1906-07) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: human hepatoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(<a href="/products/ER1906-07" style="font-weight: bold;text-decoration: underline;">ER1906-07</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: human hepatoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-P21 Polyclonal Antibody, Unconjugated(ER1906-07) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Antibody incubation with (CDKN1A/P21) Polyclonal Antibody, Unconjugated (<a href="/products/ER1906-07" style="font-weight: bold;text-decoration: underline;">ER1906-07</a>) 1:100, 90 minutes at 37℃; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37℃ for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Antibody incubation with (CDKN1A/P21) Polyclonal Antibody, Unconjugated (ER1906-07) 1:100, 90 minutes at 37℃; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37℃ for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-TNFR1/FITC Conjugated antibody (<a href="/products/ER1906-07" style="font-weight: bold;text-decoration: underline;">ER1906-07</a> /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

    Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-TNFR1/FITC Conjugated antibody (ER1906-07 /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

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metafield image

CDKN1A/P21 Rabbit Polyclonal Antibody

Application: WB,IHC-P

Reactivity: Human,Mouse

Conjugate: unconjugated