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CHD1 Mouse Monoclonal Antibody [2-2]

Usd: 350

Specification

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Catalog# M1211-5

CHD1 Mouse Monoclonal Antibody [2-2]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

CHD1 Mouse Monoclonal Antibody [2-2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human CHD1 aa 1,479-1,710 / 1,710.

Species Reactivity

Human, Mouse, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 197 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, MOLT-4 cell lysate, COS-1 cell lysate, mouse testis tissue.

Conjugation

unconjugated

Clone Number

2-2

RRID

AB_3073050

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:100

Target

Function

Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms.This family of proteins, which consists of nine members, has been divided into three separate subfamilies.All of the CHD proteins contain two tandem N-terminal chromodomains, a SWI/SNF-related ATPase domain, and a C-terminal DNA binding domain.CHD1 may play an important role in gene regulation.

Background References

1. Double chromodomains cooperate to recognize the methylated histone H3 tail."Flanagan J.F., Mi L.-Z., Chruszcz M., Cymborowski M., Clines K.L., Kim Y., Minor W., Rastinejad F., Khorasanizadeh S.Nature 438:1181-1185(2005)

2. Human but not yeast CHD1 binds directly and selectively to histone H3 methylated at lysine 4 via its tandem chromodomains."Sims R.J. III, Chen C.-F., Santos-Rosa H., Kouzarides T., J. Biol. Chem. 280:41789-41792(2005)

Sequence Similarity

Belongs to the SNF2/RAD54 helicase family.

Tissue Specificity

Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: O14646 Human

SwissProt: P40201 Mouse

Synonyms

ATP dependent helicase CHD 1 antibody

ATP-dependent helicase CHD1 antibody

CHD 1 antibody

CHD-1 antibody

Chd1 antibody

CHD1_HUMAN antibody

Chromodomain helicase DNA binding protein 1 antibody

Chromodomain-helicase-DNA-binding protein 1 antibody

DKFZp686E2337 antibody

OTTHUMP00000222625 antibody

Images

  • Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (<a href="/products/M1211-5" style="font-weight: bold;text-decoration: underline;">M1211-5</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: MOLT-4 cell lysate<br />Lane 4: COS-1 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 197 kDa<br />Observed band size: 260 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1211-5" style="font-weight: bold;text-decoration: underline;">M1211-5</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: MOLT-4 cell lysate
    Lane 4: COS-1 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 197 kDa
    Observed band size: 260 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (<a href="/products/M1211-5" style="font-weight: bold;text-decoration: underline;">M1211-5</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-CHD1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 197 kDa<br />Observed band size: 260 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1211-5" style="font-weight: bold;text-decoration: underline;">M1211-5</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-CHD1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 197 kDa
    Observed band size: 260 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis on recombinant protein using anti-CHD1 Mouse mAb (Cat. # <a href="/products/M1211-5" style="font-weight: bold;text-decoration: underline;">M1211-5</a>).

    Western blot analysis on recombinant protein using anti-CHD1 Mouse mAb (Cat. # M1211-5).

  • Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CHD1 Mouse mAb (Cat. # <a href="/products/M1211-5" style="font-weight: bold;text-decoration: underline;">M1211-5</a>).

    Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CHD1 Mouse mAb (Cat. # M1211-5).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"