CHD1 Mouse Monoclonal Antibody [2-2]
Catalog# M1211-5
CHD1 Mouse Monoclonal Antibody [2-2]
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WB
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IHC-P
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Human
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Mouse
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Green monkey
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unconjugated
Overview
Product Name
CHD1 Mouse Monoclonal Antibody [2-2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CHD1 aa 1,479-1,710 / 1,710.
Species Reactivity
Human, Mouse, Green monkey
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 197 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, MOLT-4 cell lysate, COS-1 cell lysate, mouse testis tissue.
Conjugation
unconjugated
Clone Number
2-2
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:100
Target
Function
Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms.This family of proteins, which consists of nine members, has been divided into three separate subfamilies.All of the CHD proteins contain two tandem N-terminal chromodomains, a SWI/SNF-related ATPase domain, and a C-terminal DNA binding domain.CHD1 may play an important role in gene regulation.
Background References
1. Double chromodomains cooperate to recognize the methylated histone H3 tail."Flanagan J.F., Mi L.-Z., Chruszcz M., Cymborowski M., Clines K.L., Kim Y., Minor W., Rastinejad F., Khorasanizadeh S.Nature 438:1181-1185(2005)
2. Human but not yeast CHD1 binds directly and selectively to histone H3 methylated at lysine 4 via its tandem chromodomains."Sims R.J. III, Chen C.-F., Santos-Rosa H., Kouzarides T., J. Biol. Chem. 280:41789-41792(2005)
Sequence Similarity
Belongs to the SNF2/RAD54 helicase family.
Tissue Specificity
Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
ATP dependent helicase CHD 1 antibody
ATP-dependent helicase CHD1 antibody
CHD 1 antibody
CHD-1 antibody
Chd1 antibody
CHD1_HUMAN antibody
Chromodomain helicase DNA binding protein 1 antibody
Chromodomain-helicase-DNA-binding protein 1 antibody
DKFZp686E2337 antibody
OTTHUMP00000222625 antibody
Images
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Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MOLT-4 cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 197 kDa
Observed band size: 260 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CHD1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 197 kDa
Observed band size: 260 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CHD1 Mouse mAb (Cat. # M1211-5).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"