CHRNA7 Recombinant Rabbit Monoclonal Antibody [PSH19-20]
Catalog# HA724054
CHRNA7 Recombinant Rabbit Monoclonal Antibody [PSH19-20]
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WB
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IHC-P
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IHC-Fr
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IF-Cell
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FC
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Human
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Mouse
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Rat
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HA751699
不含抗保成分
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unconjugated
Overview
Product Name
CHRNA7 Recombinant Rabbit Monoclonal Antibody [PSH19-20]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CHRNA7 aa 290-502.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IHC-Fr, IF-Cell, FC
Molecular Weight
Predicted band size: 56 kDa
Positive Control
Jurkat cell lysate, HeLa cell lysate, A431 cell lysate, Caco-2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate, Rat spleen tissue lysate, mouse dorsal root ganglion tissue, rat dorsal root ganglion tissue, Jurkat, PC-12.
Conjugation
unconjugated
Clone Number
PSH19-20
Reactivity Data
| WB | IHC-P | IHC-Fr | IF-Cell | FC | |
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| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:1,000
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IHC-Fr
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1:500
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IF-Cell
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1:500
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FC
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1:1,000
Target
Function
Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators.CHRNA7 forms homopentameric neuronal acetylcholine receptors abundantly expressed in the central nervous system, characterized by fast desensitization and high calcium permeability.In T cells, activation induces metabotropic signaling that results in an increase of intracellular Ca2+ concentrations, independent of ionotropic receptor functions (PubMed:17709503). In macrophages, required for acetylcholine-mediated inhibition of TNF and other inflammatory cytokine release
Background References
1. Ellison M et al. Alpha-conotoxins ImI and ImII target distinct regions of the human alpha7 nicotinic acetylcholine receptor and distinguish human nicotinic receptor subtypes. Biochemistry. 2004 Dec.
2. Wang H et al. Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature. 2003 Jan.
Subcellular Location
Postsynaptic cell membrane, cell membrane
Synonyms
7nAChR antibody
a7 nicotinic acetylcholine receptor antibody
acetylcholine receptor neuronal nicotinic alpha-7 subunit antibody
ACHA7_HUMAN antibody
AChR antibody
Acra7 antibody
alpha 7 neuronal nicotinic acetylcholine receptor antibody
alpha-7 nicotinic cholinergic receptor subunit antibody
alpha7 antibody
cholinergic receptor neuronal nicotinic alpha polypeptide 7 antibody
ExpandImages
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Western blot analysis of CHRNA7 on different lysates with Rabbit anti-CHRNA7 antibody (HA724054) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate (no heat) (20 µg/Lane)
Lane 2: HeLa cell lysate (no heat) (20 µg/Lane)
Lane 3: A431 cell lysate (no heat) (20 µg/Lane)
Lane 4: Caco-2 cell lysate (no heat) (20 µg/Lane)
Lane 5: RAW264.7 cell lysate (no heat) (20 µg/Lane)
Lane 6: C6 cell lysate (no heat) (20 µg/Lane)
Lane 7: PC-12 cell lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 56 kDa
Observed band size: 60/48 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724054) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CHRNA7 on different lysates with Rabbit anti-CHRNA7 antibody (HA724054) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate (no heat) (20 µg/Lane)
Lane 2: C6 cell lysate (no heat) (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (no heat) (20 µg/Lane)
Lane 5: Rat brain tissue lysate (no heat) (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 56 kDa
Observed band size: 60/48 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724054) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion tissue with Rabbit anti-CHRNA7 antibody (HA724054) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724054) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue with Rabbit anti-CHRNA7 antibody (HA724054) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724054) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IHC-Fr
Species: Rat
Site: Kidney
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunocytochemistry analysis of Jurkat cells labeling CHRNA7 with Rabbit anti-CHRNA7 antibody (HA724054) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHRNA7 antibody (HA724054) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling CHRNA7 with Rabbit anti-CHRNA7 antibody (HA724054) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHRNA7 antibody (HA724054) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of Jurkat cells labeling CHRNA7.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA724054, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of PC-12 cells labeling CHRNA7.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA724054, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"