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CLIC2 Mouse Monoclonal Antibody [A5G9]

Usd: 350

Specification

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Catalog# HA600063

CLIC2 Mouse Monoclonal Antibody [A5G9]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CLIC2 Mouse Monoclonal Antibody [A5G9]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human CLIC2 aa 50-247 / 247.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

28 kDa

Positive Control

K-562 (Human chronic myelogenous leukemia cell) cell lysate, HepG2, human fetal skeletal muscle tissue, human liver tissue, SH-SY5Y.

Conjugation

unconjugated

Clone Number

A5G9

RRID

AB_3071680

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:5,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:100-1:500

Target

Function

Chloride intracellular channel protein 2 is a protein that in humans is encoded by the CLIC2 gene. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 2 is a member of the p64 family; the protein is detected in fetal liver and adult skeletal muscle tissue. This gene maps to the candidate region on chromosome X for incontinentia pigmenti. This protein plays a role in inhibiting the function of ryanodine receptor 2. A mutation in this gene is the cause of an X-linked form of cognitive disability.

Background References

1. Zeng J. et. al. Tilapia and human CLIC2 structures are highly conserved. Biochem Biophys Res Commun. 2018 Jan

2. Syahidatulamali CS. et. al. Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients. J Postgrad Med. 2017 Oct-Dec

Subcellular Location

Cytoplasm, Membrane.

UNIPROT

SwissProt: O15247 Human

Synonyms

Chloride intracellular channel 2 antibody

Chloride intracellular channel protein 2 antibody

CLIC 2b antibody

CLIC2 antibody

CLIC2_HUMAN antibody

CLIC2b antibody

MRXS32 antibody

OTTHUMP00000196471 antibody

XAP 121 antibody

XAP121 antibody

Images

  • Western blot analysis of CLIC2 on K-562 (Human chronic myelogenous leukemia cell) cell lysate with Rabbit anti-CLIC2 antibody (<a href="/products/HA600063" style="font-weight: bold;text-decoration: underline;">HA600063</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 4 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA600063" style="font-weight: bold;text-decoration: underline;">HA600063</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa

    Western blot analysis of CLIC2 on K-562 (Human chronic myelogenous leukemia cell) cell lysate with Rabbit anti-CLIC2 antibody (HA600063) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 4 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA600063, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • ICC staining of CLIC2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA600063" style="font-weight: bold;text-decoration: underline;">HA600063</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of CLIC2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600063, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CLIC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA600063" style="font-weight: bold;text-decoration: underline;">HA600063</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CLIC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600063, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CLIC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA600063" style="font-weight: bold;text-decoration: underline;">HA600063</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CLIC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600063, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of CLIC2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA600063" style="font-weight: bold;text-decoration: underline;">HA600063</a>, 1ug/ml) (red) compared with Mouse IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of CLIC2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600063, 1ug/ml) (red) compared with Mouse IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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