COL1A2 Recombinant Rabbit Monoclonal Antibody [PSH07-21]
Overview
Product Name
COL1A2 Recombinant Rabbit Monoclonal Antibody [PSH07-21]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human COL1A2 aa 1,081-1,366.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 129 kDa
Positive Control
T98G cell lysate, Rat skin tissue lysate, T98G.
Conjugation
unconjugated
Clone Number
PSH07-21
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
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| Rat |
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|
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| Mouse |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:100
-
FC
-
1:1,000
Target
Function
Collagen alpha-2(I) chain is a protein that in humans is encoded by the COL1A2 gene. This gene encodes one of the chains for type I collagen, the fibrillar collagen found in most connective tissues. Mutations in this gene are associated with osteogenesis imperfecta, Cardiac-valvular, and Arthrochlasia type Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for alpha-1 type I collagen, since alpha-2 is less abundant. Multiple messages for this gene result from multiple polyadenylation signals, a feature shared by most of the other collagen genes.
Background References
1. Huang K et al. STAT4 and COL1A2 are potential diagnostic biomarkers and therapeutic targets for heart failure comorbided with depression. Brain Res Bull. 2022 Jun
2. Wang Y et al. COL1A2 inhibition suppresses glioblastoma cell proliferation and invasion. J Neurosurg. 2022 Aug
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Synonyms
Alpha 2 collagen type I antibody
Alpha 2 type I collagen antibody
Alpha 2 type I procollagen antibody
Alpha 2(I) collagen antibody
Alpha 2(I) procollagen antibody
Alpha-2 type I collagen antibody
CO1A2_HUMAN antibody
COL1A2 antibody
Collagen alpha 2(I) chain antibody
Collagen alpha-2(I) chain antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of COL1A2 on different lysates with Rabbit anti-COL1A2 antibody (HA722809) at 1/2,000 dilution.
Lane 1: T98G cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (negative) (20 µg/Lane)
Lane 3: Rat skin tissue lysate (40 µg/Lane)
Predicted band size: 129 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722809) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of T98G (positive) and HeLa (negative) labeling COL1A2 with Rabbit anti-COL1A2 antibody (HA722809) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COL1A2 antibody (HA722809) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
Flow cytometric analysis of HeLa (left, negative) and T98G (right, positive) cells labeling COL1A2.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722809, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Development of ECM-inspired supramolecular cryogels with innate mineralization and compression-resistance for 3D culture of mini-bone trabeculae tissue analogs
Journal: Biofabrication
DOI: 10.1088/1758-5090/ae67a5
IF: 8.2
Application: IF-tissue
Reactivity: Rat
Publish date: 2026 May
-
THOC6 deficiency leads to Cardiomyopathy by Reducing Myocardial Contractile Proteins in Cardiomyocytes
Journal: Experimental Cell Research
DOI: 10.1016/j.yexcr.2026.115025
IF: 3.5
Application: WB,IF-cell
Reactivity: Rat,Human
Publish date: 2026 Apr
-
miR‑27b‑3p modulates CD4+CD39+ Tregs to drive immune‑mediated intervertebraldisc degeneration
Journal: International Journal Of Molecular Medicine
DOI: 10.3892/ijmm.2025.5634
IF: 5.8
Application: WB
Reactivity:
Publish date: 2025 Sept
-
Therapeutic potential of miR-204-5p in intervertebral disc degeneration: targeting the SSRP1/NF-κB pathway to inhibit apoptosis
Journal: Journal Of Orthopaedic Surgery And Research
DOI: 10.1186/s13018-025-05999-3
IF: 2.8
Application: WB
Reactivity: Rat
Publish date: 2025 Jun
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