CRMP4 Recombinant Rabbit Monoclonal Antibody [JE55-99]
Catalog# HA721926
CRMP4 Recombinant Rabbit Monoclonal Antibody [JE55-99]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CRMP4 Recombinant Rabbit Monoclonal Antibody [JE55-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CRMP4 aa 451-500 / 570.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 62 kDa
Positive Control
SH-SY5Y cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE55-99
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Enables filamin binding activity. Predicted to be involved in several processes, including actin filament organization; regulation of plasma membrane bounded cell projection organization; and response to axon injury. Predicted to act upstream of or within nervous system development. Predicted to be located in several cellular components, including cell body; growth cone; and lamellipodium. Predicted to be part of filamentous actin. Predicted to be active in synapse. Predicted to colocalize with exocytic vesicle. Eecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Background References
1. Oya H., Kanda M., Sugimoto H., Shimizu D., Takami H., Hibino S., Hashimoto R., Okamura Y., Yamada S., Kodera Y. Dihydropyrimidinase-like 3 is a putative hepatocellular carcinoma tumor suppressor. J Gastroenterol 50:590-600 (2015).
2. Ponnusamy R., Lebedev A.A., Pahlow S., Lohkamp B. Crystal structure of human CRMP-4: correction of intensities for lattice- translocation disorder. Acta Crystallogr D Biol Crystallogr 70:1680-1694 (2014).
Subcellular Location
Cytoplasm. Cell projection.
Synonyms
Collapsin response mediator protein 4 antibody
Collapsin response mediator protein 4 long antibody
CRMP 4 antibody
CRMP-4 antibody
CRMP4 antibody
Dihydropyrimidinase like 3 antibody
Dihydropyrimidinase related protein 3 antibody
Dihydropyrimidinase-related protein 3 antibody
DPYL3_HUMAN antibody
DPYSL3 antibody
ExpandImages
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Western blot analysis of CRMP4 on different lysates with Rabbit anti-CRMP4 antibody (HA721926) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721926) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CRMP4 on different lysates with Rabbit anti-CRMP4 antibody (HA721926) at 1/1,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si CRMP4 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721926) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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