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CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH24-56] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751946

CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH24-56] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • FC

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH24-56] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CSPG4 aa 151-600.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC, IP

Molecular Weight

Predicted band size: 251 kDa

Positive Control

A375 (Human amelanotic melanoma cells) cell lysate, SK-MEL-28 (Human malignant melanoma cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, Mouse brain tissue lysate, Mouse spinal cord tissue lysate, Mouse pancreas tissue lysate, Rat brain tissue lysate, Rat pancreas tissue lysate.

Conjugation

unconjugated

Clone Number

PSH24-56

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:200-1:5,000

  • FC

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. CSPG4/NG2 is also a hallmark protein of oligodendrocyte progenitor cells (OPCs)[8] and OPC dysfunction has been implicated as a candidate pathophysiological mechanism of familial schizophrenia.

Background References

1. Greiner D et al. Human CSPG4-targeting CAR-macrophages inhibit melanoma growth. Oncogene. 2025 Jun

2. Chen X et al. Chondroitin Sulfate Proteoglycan 4 (CSPG4) as an Emerging Target for Immunotherapy to Treat Melanoma. Cancers (Basel). 2024 Sep

Subcellular Location

Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface.

UNIPROT

SwissProt: Q6UVK1 Human

SwissProt: Q8VHY0 Mouse

SwissProt: Q00657 Rat

Synonyms

4732461B14Rik antibody

AN2 antibody

AN2 proteoglycan antibody

Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody

Chondroitin sulfate proteoglycan 4 antibody

Chondroitin sulfate proteoglycan NG2 antibody

Cspg4 antibody

Cspg4 chondroitin sulfate proteoglycan 4 antibody

CSPG4_HUMAN antibody

HMW-MAA antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (<a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>) at 1/5,000 dilution. <br /> <br />Lane 1: A375 (Human amelanotic melanoma cells) cell lysate <br />Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate <br />Lane 3: MCF7 (Human breast cancer cells) cell lysate <br />Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate <br />Lane 5: SK-BR-3 (Human breast cancer cells) cell lysate <br />Lane 6: NIH/3T3 (Mouse fibroblasts) cell lysate <br />Lane 7: N9 (Mouse microglial cells) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 seconds; ECL: K1801 <br /> <br />MCF7 is a negative control (PMID: 20852124). <br />SK-BR-3 is a negative control (PMID: 20852124). <br />Negative expression of Cspg4 protein in N9 cells is consistent with the predicted expression pattern. <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 251 kDa <br />Observed band size: 330 kDa

    ☑ Relative expression (RE)

    Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (HA751946) at 1/5,000 dilution.

    Lane 1: A375 (Human amelanotic melanoma cells) cell lysate
    Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate
    Lane 3: MCF7 (Human breast cancer cells) cell lysate
    Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate
    Lane 5: SK-BR-3 (Human breast cancer cells) cell lysate
    Lane 6: NIH/3T3 (Mouse fibroblasts) cell lysate
    Lane 7: N9 (Mouse microglial cells) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 seconds; ECL: K1801

    MCF7 is a negative control (PMID: 20852124).
    SK-BR-3 is a negative control (PMID: 20852124).
    Negative expression of Cspg4 protein in N9 cells is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751946, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 251 kDa
    Observed band size: 330 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (<a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Mouse spinal cord tissue lysate<br />Lane 4: Mouse pancreas tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 15 seconds; ECL: K1801<br />Mouse liver is a negative control (PMID: 23481707).<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 251 kDa<br />Observed band size: 330 kDa

    ☑ Relative expression (RE)

    Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (HA751946) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Mouse spinal cord tissue lysate
    Lane 4: Mouse pancreas tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 15 seconds; ECL: K1801
    Mouse liver is a negative control (PMID: 23481707).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751946, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 251 kDa
    Observed band size: 330 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (<a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>) at 1/5,000 dilution. <br /> <br />Lane 1: Rat brain tissue lysate <br />Lane 2: Rat liver tissue lysate <br />Lane 3: Rat pancreas tissue lysate <br /> <br />Lysates/proteins at 30 µg/Lane. <br />Exposure time: 2 minutes; ECL: K1801 <br /> <br />Rat liver is a negative control (PMID: 23481707). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 251 kDa <br />Observed band size: 330 kDa

    ☑ Relative expression (RE)

    Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (HA751946) at 1/5,000 dilution.

    Lane 1: Rat brain tissue lysate
    Lane 2: Rat liver tissue lysate
    Lane 3: Rat pancreas tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 2 minutes; ECL: K1801

    Rat liver is a negative control (PMID: 23481707).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751946, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 251 kDa
    Observed band size: 330 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br /> <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br />Primary antibody: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>, 1/5,000, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br /> <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). <br />Negative control: SK-Br-3 (PMID: 20852124).

    ☑ Relative expression (RE)

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: HA751946, 1/5,000, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

    Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
    Negative control: SK-Br-3 (PMID: 20852124).

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Mouse <br />Sample: NIH/3T3 (Mouse fibroblasts) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br /> <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br />Primary antibody: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br /> <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblasts)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: HA751946, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

    Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Flow Cytometry <br /> <br />Species: Human <br />Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells) <br /> <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br />Primary antibody: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a> (1/1,000) (Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br />Negative control: SK-Br-3 (PMID: 20852124).

    ☑ Relative expression (RE)

    Application: Flow Cytometry

    Species: Human
    Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells)

    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.
    Primary antibody: HA751946 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
    Negative control: SK-Br-3 (PMID: 20852124).

  • Application: Flow Cytometry<br /><br />Species: Mouse<br />Sample: NIH/3T3 (Mouse fibroblasts)<br /><br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS.<br />Primary antibody: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a> (1/1,000) (Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblasts)

    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.
    Primary antibody: HA751946 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

  • Immunoprecipitation (IP)<br /><br />CSPG4 was immunoprecipitated in 0.2 mg SK-MEL-28 (Human malignant melanoma cell) cell lysate with <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a> at 1/20,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: SK-MEL-28 cell lysate (input)<br />Lane 2: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a> IP in SK-MEL-28 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a> in SK-MEL-28 cell lysate<br /><br />Exposure time: 10 seconds<br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br /><br />Primary dilution: <a href="/products/HA751946" style="font-weight: bold;text-decoration: underline;">HA751946</a>, 1/20,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature<br /><br />Predicted band size: 251 kDa<br />Observed band size: 330 kDa

    Immunoprecipitation (IP)

    CSPG4 was immunoprecipitated in 0.2 mg SK-MEL-28 (Human malignant melanoma cell) cell lysate with HA751946 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751946 at 1/20,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: SK-MEL-28 cell lysate (input)
    Lane 2: HA751946 IP in SK-MEL-28 cell lysate
    Lane 3: Rabbit IgG instead of HA751946 in SK-MEL-28 cell lysate

    Exposure time: 10 seconds
    Blocking: 5% NFDM/TBST, 1 hour at room temperature

    Primary dilution: HA751946, 1/20,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 251 kDa
    Observed band size: 330 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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