CXCR1 Rabbit Polyclonal Antibody
Catalog# HA500526
CXCR1 Rabbit Polyclonal Antibody
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WB
-
IF-Cell
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Human
-
unconjugated
Overview
Product Name
CXCR1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within full length human CXCR1 protein.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size:
Positive Control
U87-MG cell lysate, Hela cell lysate, Jurkat cell lysate, HepG2 cell lysate, Hela.
Conjugation
unconjugated
RRID
Reactivity Data
| WB | IF-Cell | |
|---|---|---|
| Human |
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IF-Cell
-
1:200
Target
Function
Interleukin 8 receptor, alpha is a chemokine receptor. This name and the corresponding gene symbol IL8RA have been replaced by the HGNC approved name C-X-C motif chemokine receptor 1 and the approved symbol CXCR1. It has also been designated as CD181 (cluster of differentiation 181). The IUPHAR Committee on Receptor Nomenclature and Drug Classification use the HGNC recommended name, CXCR1. The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein-activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. IL8RA, IL8RB, which encodes another high affinity IL8 receptor, and IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Stimulation of CXCR1 in neutrophils by its primary ligand, Interleukin 8, leads to neutrophil chemotaxis and activation.
Background References
1. Teijeira Á et al. CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune Cytotoxicity. Immunity. 2020 May
2. Tang KH et al. Combined Inhibition of SHP2 and CXCR1/2 Promotes Antitumor T-cell Response in NSCLC. Cancer Discov. 2022 Jan
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
C C antibody
C C CKR 1 antibody
C-X-C chemokine receptor type 1 antibody
CCCKR1 antibody
CD 181 antibody
CD128 antibody
CD181 antibody
CD181 antigen antibody
CDw128a antibody
Chemokine (C X C motif) receptor 1 antibody
ExpandImages
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Western blot analysis of CXCR1 on different lysates with Rabbit anti-CXCR1 antibody (HA500526) at 1/1,000 dilution.
Lane 1: U87-MG cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 38 kDa
Exposure time: 50 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling CXCR1 with Rabbit anti-CXCR1 antibody (HA500526) at 10μg/mL dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CXCR1 antibody (HA500526) at 10μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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