CXCR2 Recombinant Rabbit Monoclonal Antibody [PSH21-28] - BSA and Azide free
Catalog# HA751793
CXCR2 Recombinant Rabbit Monoclonal Antibody [PSH21-28] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CXCR2 Recombinant Rabbit Monoclonal Antibody [PSH21-28] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CXCR2 aa 316-360.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
A549 cell lysate, MDA-MB-231 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, 4T1 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH21-28
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:10,000
Target
Function
Interleukin 8 receptor, beta is a chemokine receptor. IL8RB is also known as CXCR2, and CXCR2 is now the IUPHAR Committee on Receptor Nomenclature and Drug classification-recommended name. The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein-activated second messenger system (Gi/o-coupled). This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. In addition, it binds ligands CXCL2, CXCL3, and CXCL5. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. IL8RB, IL8RA, which encodes another high affinity IL8 receptor, and IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Mutations in CXCR2 cause hematological traits.
Background References
1. Leslie J et al. CXCR2 inhibition enables NASH-HCC immunotherapy. Gut. 2022 Apr
2. Cheng Y et al. Targeting CXCR2 inhibits the progression of lung cancer and promotes therapeutic effect of cisplatin. Mol Cancer. 2021 Apr
Subcellular Location
Cell membrane.
Synonyms
C-X-C chemokine receptor type 2 antibody
CD 182 antibody
CD182 antibody
CD182 antigen antibody
CDw128b antibody
Chemokine (CXC) receptor 2 antibody
CMKAR2 antibody
CXC-R2 antibody
CXCR 2 antibody
CXCR-2 antibody
ExpandImages
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Western blot analysis of CXCR2 on different lysates with Rabbit anti-CXCR2 antibody (HA751793) at 1/10,000 dilution.
Lane 1: A549 cell lysate (no heat) (10 µg/Lane)
Lane 2: MDA-MB-231 cell lysate (no heat) (10 µg/Lane)
Lane 3: SK-Br-3 cell lysate (10 µg/Lane)
Lane 4: HepG2 cell lysate (10 µg/Lane)
Lane 5: Neuro-2a cell lysate (no heat) (10 µg/Lane)
Lane 6: 4T1 cell lysate (10 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)
Lane 8: C6 cell lysate (10 µg/Lane)
Lane 9: Mouse spleen tissue lysate (no heat) (20 µg/Lane)
Lane 10: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 11: Rat spleen tissue lysate (no heat) (20 µg/Lane)
Lane 12: Rat brain tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 41 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751793) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CXCR2 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
