CXCR5 Recombinant Rabbit Monoclonal Antibody [JB11-40] - BSA and Azide free
Catalog# HA750463
CXCR5 Recombinant Rabbit Monoclonal Antibody [JB11-40] - BSA and Azide free
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WB
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IHC-P
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FC
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IF-Cell
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Human
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Mouse
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Rat
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ET7107-74
含抗保成分
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unconjugated
Overview
Product Name
CXCR5 Recombinant Rabbit Monoclonal Antibody [JB11-40] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within C-terminal human CXCR5.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC, IF-Cell
Molecular Weight
Predicted band size: 42 kDa
Positive Control
Daudi cell lysate, EL4 cell lysate, C6 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Daudi, rat kidney tissue, mouse heart tissue, Daudi.
Conjugation
unconjugated
Clone Number
JB11-40
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IF-Cell
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1:250
Target
Function
Burkitt's lymphoma receptor 1 (Blr1) is a lymphocyte specific chemokine receptor expressed at low levels in secondary lymphoid tissues and in defined structures of the cerebellum. The G-protein coupled receptor has significant homology to other chemokine receptors. Stimulation of Blr1 by its ligand, B-lymphocyte chemo-attractant (BLC) results in an influx of calcium into the cell and the chemotaxis of the cell. Blr1 is required for B-cell migration into splenic and Peyer's patch follicles. BLC expression in Peyer's patches is highest in germinal centers, where B cells undergo somatic mutation and affinity maturation.
Background References
1. Sjoeblom T et al. The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274 (2006).
2. Barella L et al. Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation. Biochem J 309:773-779 (1995).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Expression in mature B-cells and Burkitt lymphoma cells.
Subcellular Location
Cell membrane.
Synonyms
BLR 1 antibody
BLR1 antibody
Burkitt lymphoma receptor 1 antibody
Burkitt lymphoma receptor 1 GTP binding protein antibody
Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody
C X C chemokine receptor type 5 antibody
C-X-C chemokine receptor type 5 antibody
CD 185 antibody
CD185 antibody
CD185 antigen antibody
ExpandImages
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Western blot analysis of CXCR5 on different lysates with Rabbit anti-CXCR5 antibody (HA750463) at 1/2,000 dilution.
Lane 1: Daudi cell lysate
Lane 2: EL4 cell lysate
Lane 3: C6 cell lysate
Lane 4: Mouse spleen tissue lysate
Lane 5: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute 34 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750463) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Daudi cells labeling CXCR5 with Rabbit anti-CXCR5 antibody (HA750463) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCR5 antibody (HA750463) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CXCR5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750463, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CXCR5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750463, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Daudi cells labeling CXCR5.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750463, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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