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CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-04]

Usd: 385

Specification

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Catalog# HA724746

CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-04]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CYP2E1 aa 251-493.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, FC(Intra)

Target Molecular Weight

Predicted band size: 57 kDa

Positive Control

Mouse liver tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH28-04

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC(Intra) IF-Tissue
human
Tested Tested
Tested Tested
Tested Tested
 
mouse
Tested Tested
Tested Tested
Tested Tested
Tested Tested
rat
Tested Tested
 
 
 

Product Features

Form

Liquid

Concentration

0.5 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • IHC-P

  • 1:500-1:5,000

  • IF-Tissue

  • 1:100

  • FC(Intra)

  • 1:500

Target

Function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) .Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).

Background References

1. Gi M et al. Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-Dichloropropane in Male Syrian Hamsters and B6C3F1 Mice. Toxicol Sci 145:196-208 (2015).

2. Boussadia B et al. Effect of status epilepticus and antiepileptic drugs on CYP2E1 brain expression. Neuroscience 281C:124-134 (2014).

Subcellular Location

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.

UNIPROT

SwissProt: P05181 Human

SwissProt: Q05421 Mouse

SwissProt: P05182 Rat

Synonyms

4-nitrophenol 2-hydroxylase antibody

CP2E1_HUMAN antibody

CPE1 antibody

CYP2E antibody

Cyp2e1 antibody

CYPIIE1 antibody

Cytochrome P450 2E1 antibody

Cytochrome P450 isozyme 3A antibody

Cytochrome P450 LM3A antibody

Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CYP2E1 on different lysates with Rabbit anti-CYP2E1 antibody (<a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a>) at 1/10,000 dilution. <br /> <br />Lane 1: Mouse liver tissue lysate <br />Lane 2: Mouse spleen tissue lysate <br />Lane 3: Rat liver tissue lysate <br />Lane 4: Rat spleen tissue lysate <br /> <br />Lysates/proteins at 10 µg/Lane. <br />Exposure time: 30 seconds; ECL: K1801 <br /> <br />Low expression: Spleen, colon (PMID: 15576447). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 57 kDa <br />Observed band size: 50 kDa

    ☑ Relative expression (RE)

    Western blot analysis of CYP2E1 on different lysates with Rabbit anti-CYP2E1 antibody (HA724746) at 1/10,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Rat liver tissue lysate
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Low expression: Spleen, colon (PMID: 15576447).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724746, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 57 kDa
    Observed band size: 50 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724746, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724746, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724746, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724746, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • Application: Flow Cytometry (Intra) <br /> <br />Species: Human <br />Sample: MCF7 (Human breast cancer cells) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a> (1/500, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: MCF7 (Human breast cancer cells)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724746 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

  • Application: Flow Cytometry (Intra) <br /> <br />Species: Mouse <br />Sample: B16-F1 (Mouse melanoma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724746" style="font-weight: bold;text-decoration: underline;">HA724746</a> (1/500, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />

    Application: Flow Cytometry (Intra)

    Species: Mouse
    Sample: B16-F1 (Mouse melanoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724746 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"