iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
Catalog# HA720153F
iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
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IF-Cell
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FC
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Human
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Calretinin aa 60-100.
Species Reactivity
Human
Validated Applications
IF-Cell, FC
Molecular Weight
Predicted band size: 32 kDa
Positive Control
SH-SY5Y.
Conjugation
iFluor™ 488
Clone Number
JM12-93
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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FC
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1:500-1:1,000
Target
Function
Calbindin D28K and Calretinin (also designated CR or 29 kDa Calbindin) are two closely related intracellular calcium-binding proteins belonging to the Troponin-C superfamily. Initially isolated from chick retina, Calretinin shares 58% identical residues with human Calbindin D28K. Calretinin is expressed in the brain and is particularly abundant in auditory neurons with precisely timed discharges. Neurons in the nucleus accumbens containing Calretinin all possess nuclear indentations. Calretinin-immunoreactive boutons form asymmetrical and symmetrical synaptic specializations on spines, dendrites and somata. The symmetrical synaptic specializations have medium-sized spiny neurons and contact other Calretinin-immunoreactive somata. Calretinin is widely used as a immunocytochemical marker for mesothelioma.
Background References
1. Francavilla C et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017).
2. McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016).
Subcellular Location
Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.
UNIPROT
Synonyms
29 kDa calbindin antibody
CAB 29 antibody
CAB29 antibody
CAL 2 antibody
CAL2 antibody
CALB 2 antibody
CALB2 antibody
CALB2_HUMAN antibody
Calbindin 2 29kDa antibody
Calbindin 2 antibody
ExpandImages
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Immunocytochemistry analysis of SH-SY5Y cells labeling Calretinin with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. -
Flow cytometric analysis of SH-SY5Y cells labeling Calretinin.
Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Calretinin (HA720153F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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