Cannabinoid receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH02-43] - BSA and Azide free
Catalog# HA750808
Cannabinoid receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH02-43] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Cannabinoid receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH02-43] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Cannabinoid receptor 1 aa 1-472 /472.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 53 kDa
Positive Control
Human brain tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, rat cerebellum tissue lysate, mouse brain tissue, mouse hippocampus tissue, rat brain tissue, rat hippocampus tissue.
Conjugation
unconjugated
Clone Number
PSH02-43
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The endocannabinoid system consists of the cannabinoid receptors, CB1 and CB2 receptors, the enzymes that produce and degrade the endogenous cannabinoid ligands (such as FAAH, DAG lipases, and MAG lipase), and the endocannabinoid ligands derived from the metabolism of arachidonic acid, 2-arachidonoylglycerol (2-AG) and anandamide . CB1 receptor belongs to the superfamily of G protein-coupled receptors (GPCRs) and harbors a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular tail. CB1 receptor is coupled to the Gai/o subunit of the G protein which inhibits adenylyl cyclases and regulates calcium and potassium ion channels . CB1 receptor is one of the most abundant GPCRs in the central nervous system. It has been show to play critical roles in the wiring of the brain during development , in neuronal plasticity, analgesia, drug abuse and metabolic homeostasis (4). In addition, CB1 receptor has been shown to interact with other GPCRs, to give rise to novel pharmacological and signaling heteromers with implication in diseases .
Background References
1. Rozenfeld R, Bushlin I, Gomes I, Tzavaras N, Gupta A, Neves S, Battini L, Gusella GL, Lachmann A, Ma\'ayan A, Blitzer RD, Devi LA. Receptor heteromerization expands the repertoire of cannabinoid signaling in rodent neurons. PLoS One. 2012;7(1):e29239. doi: 10.1371/journal.pone.0029239. Epub 2012 Jan 3.
2. Rozenfeld R, Gupta A, Gagnidze K, Lim MP, Gomes I, Lee-Ramos D, Nieto N, Devi LA. AT1R-CB₁R heteromerization reveals a new mechanism for the pathogenic properties of angiotensin II. EMBO J. 2011 May 3;30(12):2350-63. doi: 10.1038/emboj.2011.139.
Subcellular Location
Cell membrane, Membrane raft, Mitochondrion outer membrane, Cell projection, axon, Presynapse.
Synonyms
CANN6 antibody
Cannabinoid receptor 1 antibody
CB-R antibody
CB1 antibody
CB1A antibody
CB1K5 antibody
CB1R antibody
Central cannabinoid receptor antibody
CNR antibody
CNR1 antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA721820, the same antibody clone in a different buffer formulation.
Western blot analysis of Cannabinoid receptor 1 on different lysates with Rabbit anti-Cannabinoid receptor 1 antibody (HA721820) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Human liver tissue lysate (negative control)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse cerebellum tissue lysate
Lane 5: Mouse liver tissue lysate (negative control)
Lane 6: Rat brain tissue lysate
Lane 7: Rat cerebellum tissue lysate
Lane 8: Rat liver tissue lysate (negative control)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 42 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721820) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721820, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Cannabinoid receptor 1 antibody (HA721820) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721820) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721820, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Cannabinoid receptor 1 antibody (HA721820) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721820) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA721820, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver (negative control) tissue with Rabbit anti-Cannabinoid receptor 1 antibody (HA721820) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721820) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721820, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Cannabinoid receptor 1 antibody (HA721820) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721820) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721820, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-Cannabinoid receptor 1 antibody (HA721820) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721820) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA721820, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver (negative control) tissue with Rabbit anti-Cannabinoid receptor 1 antibody (HA721820) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721820) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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