Casein Kinase 1 alpha Recombinant Rabbit Monoclonal Antibody [PSH04-46] - BSA and Azide free
Catalog# HA750955
Casein Kinase 1 alpha Recombinant Rabbit Monoclonal Antibody [PSH04-46] - BSA and Azide free
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WB
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IF-Cell
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FC
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IP
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Human
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Mouse
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Rat
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HA722131
含抗保成分
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unconjugated
Overview
Product Name
Casein Kinase 1 alpha Recombinant Rabbit Monoclonal Antibody [PSH04-46] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CSNK1A1 aa 1-337 / 337.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC, IP
Molecular Weight
Predicted band size: 39 kDa
Positive Control
HCT 116 cell lysate, HeLa cell lysate, HEK-293 cell lysate, MCF7 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HCT 116.
Conjugation
unconjugated
Clone Number
PSH04-46
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:500
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity).
Background References
1. Ma Z et al. Knockdown of Csnk1a1 results in preimplantation developmental arrest in mice. Theriogenology. 2023 Mar
2. Pan M et al. CSNK1A1/CK1α suppresses autoimmunity by restraining the CGAS-STING1 signaling. Autophagy. 2024 Feb
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome, centromere, kinetochore, Nucleus speckle, cilium basal body, spindle.
Synonyms
Casein kinase 1 alpha 1 antibody
Casein kinase I isoform alpha antibody
CK1 antibody
CK1A antibody
CKI alpha antibody
CKI-alpha antibody
CKIa antibody
Clock regulator kinase antibody
Csnk1a1 antibody
Down regulated in lung cancer antibody
ExpandImages
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Western blot analysis of Casein Kinase 1 alpha on different lysates with Rabbit anti-Casein Kinase 1 alpha antibody (HA750955) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: MCF7 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: RAW264.7 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 34 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750955) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HCT 116 cells labeling Casein Kinase 1 alpha with Rabbit anti-Casein Kinase 1 alpha antibody (HA750955) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 1 alpha antibody (HA750955) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HCT 116 cells labeling Casein Kinase 1 alpha.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750955, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with HA750955 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750955 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA750955 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA750955 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 11 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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