Caspase-7 Recombinant Rabbit Monoclonal Antibody [PSH13-69] - BSA and Azide free
Catalog# HA751482
Caspase-7 Recombinant Rabbit Monoclonal Antibody [PSH13-69] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA723558
含抗保成分
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unconjugated
Overview
Product Name
Caspase-7 Recombinant Rabbit Monoclonal Antibody [PSH13-69] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Caspase-7 aa 1-303.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 34 kDa
Positive Control
HeLa cell lysate, HeLa treated with 1μM staurosporine for 3 hours cell lysate, C2C12 cell lysate, C2C12 treated with 1μM staurosporine for 3 hours cell lysate, human colon tissue, mouse colon tissue, rat colon tissue.
Conjugation
unconjugated
Clone Number
PSH13-69
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:200-1:1,000
Target
Function
Caspase-7, apoptosis-related cysteine peptidase, also known as CASP7, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing by upstream caspases (caspase-8, -9) at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme in the form of a heterotetramer. The precursor of this caspase is cleaved by caspase 3, caspase 10, and caspase 9. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms.
Background References
1. Nozaki K et al. Caspase-7 activates ASM to repair gasdermin and perforin pores. Nature. 2022 Jun
2. Li X et al. Apoptotic caspase-7 activation inhibits non-canonical pyroptosis by GSDMB cleavage. Cell Death Differ. 2023 Sep
Subcellular Location
Cytoplasm, cytosol, Nucleus, Secreted, extracellular space.
Synonyms
Apoptotic protease Mch-3 antibody
Apoptotic protease MCH3 antibody
CASP-7 antibody
CASP7 antibody
CASP7_HUMAN antibody
Caspase 7 antibody
Caspase 7 apoptosis related cysteine peptidase antibody
Caspase-7 subunit p11 antibody
Caspase7 antibody
CMH 1 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Caspase-7 on different lysates with Rabbit anti-Caspase-7 antibody (HA751482) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 3 hours cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C2C12 treated with 1μM staurosporine for 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34/32/20/18 kDa
Exposure time: 2 minute 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751482) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Caspase-7 antibody (HA751482) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751482) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Caspase-7 antibody (HA751482) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751482) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Caspase-7 antibody (HA751482) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751482) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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