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Caspase-8 Mouse Monoclonal Antibody [6G1]

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Catalog# RT1099

Caspase-8 Mouse Monoclonal Antibody [6G1]

Application

  • WB

  • IP

  • IF

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Caspase-8 Mouse Monoclonal Antibody [6G1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

peptide

Species Reactivity

Human

Validated Applications

WB, IP, IF, IHC-P

Molecular Weight

55kDa

Positive Control

Hela, Jurkat, CCRF-CEM, MOLT-4

Conjugation

unconjugated

Clone Number

6G1

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃

Storage Buffer

1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100-1:1,000

  • IP

  • 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

  • IF

  • 1:50-500

  • IHC-P

  • 1:50-500

Target

Function

Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.

Background References

1. Wang, O., et al. 2011. Anticancer activity of 2α, 3α, 19β, 23β-Tetrahydroxyurs-12-en-28-oic acid (THA), a novel triterpenoid isolated from Sinojackia sarcocarpa. PLoS ONE 6: e21130.

2. Cleveland, J.L., et al. 1995. Contenders in FAS-L/TNF death signaling. Cell 81: 479-482.

Subcellular Location

Cytoplasm

UNIPROT

SwissProt: Q14790 Human

Synonyms

ALPS2B antibody

Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody

Apoptosis related cysteine peptidase antibody

Apoptotic cysteine protease antibody

Apoptotic protease Mch-5 antibody

Apoptotic protease Mch5 antibody

CAP 4 antibody

CAP4 antibody

CASP-8 antibody

CASP8 antibody

Expand

Images

  • Western blot analysis of caspase-8 expression in Jurkat whole cell lysate.

    Western blot analysis of caspase-8 expression in Jurkat whole cell lysate.

  • Western blot analysis of caspase-8 expression in CCRF-CEM (A), HeLa (B) and MOLT-4 (C) whole cell lysates.

    Western blot analysis of caspase-8 expression in CCRF-CEM (A), HeLa (B) and MOLT-4 (C) whole cell lysates.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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