Caspr Mouse Monoclonal Antibody [A2D2]
Catalog# EM1902-10
Caspr Mouse Monoclonal Antibody [A2D2]
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
Caspr Mouse Monoclonal Antibody [A2D2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human Caspr aa 1100-1300.
Species Reactivity
Human, Mouse
Validated Applications
IHC-P, FC
Molecular Weight
Predicted band size: 156 kDa
Positive Control
Human kidney tissue, mouse kidney tissue, SH-SY5Y.
Conjugation
unconjugated
Clone Number
A2D2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
CASPR also known as Contactin associated protein 1, Paranodin and CASPR1 is a protein that in humans is encoded by the CNTNAP1 gene. CASPR is a part of the neurexin family of proteins, hence its another name "Neurexin IV". CASPR is a membrane protein found in the neuronal membrane in the paranodal section of the axon[[]] in myelinated neurons, between the Nodes of Ranvier containing Na+ channels, and juxtaparanode, which contains K+ channels. During myelination, caspr associates with contactin in a cis complex, though its precise role in myelination is not yet understood. The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons.
Background References
1. Bergadà-Pijuan J. et. al. CASPR, an analysis pipeline for single and paired guide RNA CRISPR screens, reveals optimal target selection for long noncoding RNAs. Bioinformatics. 2019 Nov 4.
2. Gordon A. et. al. Caspr and caspr2 are required for both radial and longitudinal organization of myelinated axons. J Neurosci. 2014 Nov 5;34(45):14820-6.
Sequence Similarity
Belongs to the neurexin family.
Tissue Specificity
Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis.
Subcellular Location
Membrane, paranodal septate junction.
Synonyms
Caspr antibody
Caspr1 antibody
CNTNAP antibody
Cntnap1 antibody
CNTP1_HUMAN antibody
Contactin associated protein 1 antibody
Contactin-associated protein 1 antibody
MHDNIV antibody
NCP1 antibody
Neurexin 4 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspr antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspr antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Caspr was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-10, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Caspr Recombinant Rabbit Monoclonal Antibody [JE31-54]
Application: WB,IHC-P,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
