Cathepsin D Recombinant Rabbit Monoclonal Antibody [JE24-31]
Catalog# ET7110-58
Cathepsin D Recombinant Rabbit Monoclonal Antibody [JE24-31]
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
Cathepsin D Recombinant Rabbit Monoclonal Antibody [JE24-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within C terminal Human Cathepsin D.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P
Molecular Weight
Predicted band size 45 kDa
Positive Control
MCF-7, human lung tissue, human liver tissue, human liver carcinoma tissue.
Conjugation
unconjugated
Clone Number
JE24-31
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:200
Target
Function
This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.
Background References
1. Suzuki C. et. al. Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury. Int J Mol Sci. 2019 Apr 5;20(7).
2. Dubey V. et. al. Cathepsin D as a Promising Target for the Discovery of Novel Anticancer Agents. Curr Cancer Drug Targets. 2017;17(5):404-422.
Sequence Similarity
Belongs to the peptidase A1 family.
Tissue Specificity
Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post-translational Modification
N- and O-glycosylated.; Undergoes proteolytic cleavage and activation by ADAM30.; As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Subcellular Location
Lysosome, extracellular space, melanosome.
UNIPROT
Synonyms
CatD antibody
CATD_HUMAN antibody
Cathepsin D antibody
Cathepsin D heavy chain antibody
CD antibody
Ceroid lipofuscinosis neuronal 10 antibody
CLN10 antibody
CPSD antibody
ctsd antibody
Epididymis secretory sperm binding protein Li 130P antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Cathepsin D antibody (ET7110-58) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-58) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cathepsin D antibody (ET7110-58) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-58) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Cathepsin D antibody (ET7110-58) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-58) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
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