Cathepsin S Recombinant Rabbit Monoclonal Antibody [PSH27-81]
Catalog# HA724726
Cathepsin S Recombinant Rabbit Monoclonal Antibody [PSH27-81]
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WB
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Human
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unconjugated
Overview
Product Name
Cathepsin S Recombinant Rabbit Monoclonal Antibody [PSH27-81]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Cathepsin S aa 1-331.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 37 kDa
Positive Control
THP-1 (Human acute monoblastic leukemia cell) cell lysate, THP-1 treated with 80nM TPA for 24 hours then treated with 100ng/mL LPS and 1μg/mL BFA for 7 hours cell lysate, RPMI8226 (Human multiple myeloma cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH27-81
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | FC(Intra) | IF-Cell | IP | |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Cathepsin S is a protein that in humans is encoded by the CTSS gene. Transcript variants utilizing alternative polyadenylation signals exist for this gene. Cathepsin S is a member of the peptidase C1 family of cysteine cathepsins, a lysosomal cysteine protease that may participate in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S can function as an elastase over a broad pH range in alveolar macrophages. Cathepsin S is a lysosomal enzyme that belongs to the papain-like protease of cysteine proteases. While a role in antigen presentation has long been recognized, it is now understood that cathepsin S has a role in itch and pain, or nociception . The nociceptive activity results from cathepsin S functioning as a signaling molecule via activation of protease-activated receptors 2 and 4 members of the G-protein coupled receptor family.
Background References
1. Dheilly E et al. Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma. Cancer Cell. 2020 May
2. McDowell SH et al. Leading the invasion: The role of Cathepsin S in the tumour microenvironment. Biochim Biophys Acta Mol Cell Res. 2020
Subcellular Location
Lysosome, Secreted, Cytoplasmic vesicle, phagosome.
UNIPROT
Synonyms
Cathepsin S antibody
CathepsinS antibody
CATS_HUMAN antibody
Ctss antibody
Images
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☑ Cell treatment (CT),Relative expression (RE)
Western blot analysis of Cathepsin S on different lysates with Rabbit anti-Cathepsin S antibody (HA724726) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 2: THP-1 treated with 80nM TPA for 24 hours, then treated with 100ng/mL LPS and 1μg/mL BFA for 7 hours cell lysate
Lane 3: RPMI8226 (Human multiple myeloma cell) cell lysate
Lane 4: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 5: MCF7 (Human breast cancer cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 46 seconds; ECL: K1801
Low expression of Cathepsin S protein in A549 or MCF7 cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724726, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37, 25 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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