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Caveolin-1 Mouse Monoclonal Antibody [19-D6]

Usd: 350

Specification

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Catalog# EM40728

Caveolin-1 Mouse Monoclonal Antibody [19-D6]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Caveolin-1 Mouse Monoclonal Antibody [19-D6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Mouse Caveolin-1 aa 129-178 / 178.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 20 kDa

Positive Control

A431 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat lung tissue lysate, mouse bladder tissue, rat lung tissue, human lung tissue, mouse lung tissue.

Conjugation

unconjugated

Clone Number

19-D6

RRID

AB_3069000

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:200-1:1,000

Target

Function

Caveolin, an integral membrane protein, is a principal component of caveolae membranes in vivo. Two isoforms of caveolin have been identified: a slower migrating 24-kDa species (-isoform) and a faster migrating 21-kDa species (-isoform). Caveolins interact with multiple signaling molecules, such as the G-protein alpha subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS. Caveolin-1 has been implicated in the pathogenesis of mammary epithelial cell hyperplasia.

Background References

1. Kaarteenaho R, et al. Epithelial N-cadherin and nuclear β-catenin are up-regulated during early development of human lung. BMC Dev Biol. 10:113 (2010)

2. Qayyum T, et al. The interrelationships between Src, Cav-1 and RhoGD12 in transitional cell carcinoma of the bladder. Br J Cancer. 106:1187-95 (2012).

Sequence Similarity

Belongs to the caveolin family.

Tissue Specificity

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

Post-translational Modification

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.; The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.; Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

Subcellular Location

Cell membrane, Golgi apparatus membrane.

UNIPROT

SwissProt: Q03135 Human

SwissProt: P49817 Mouse

SwissProt: P41350 Rat

Synonyms

BSCL3 antibody

CAV antibody

CAV1 antibody

CAV1_HUMAN antibody

caveolae protein, 22 kD antibody

caveolin 1 alpha isoform antibody

caveolin 1 beta isoform antibody

Caveolin 1 caveolae protein 22kDa antibody

Caveolin-1 antibody

Caveolin1 antibody

Expand

Images

  • Western blot analysis of Caveolin-1 on different lysates with Mouse anti-Caveolin-1 antibody (<a href="/products/EM40728" style="font-weight: bold;text-decoration: underline;">EM40728</a>) at 1/500 dilution.<br /><br />Lane 1: A431 cell lysate (20 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 3: C6 cell lysate (20 µg/Lane)<br />Lane 4: Rat lung tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: Lane 1: 3 minutes; Lane 2-4: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM40728" style="font-weight: bold;text-decoration: underline;">EM40728</a>) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caveolin-1 on different lysates with Mouse anti-Caveolin-1 antibody (EM40728) at 1/500 dilution.

    Lane 1: A431 cell lysate (20 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 3: C6 cell lysate (20 µg/Lane)
    Lane 4: Rat lung tissue lysate (30 µg/Lane)

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

    Exposure time: Lane 1: 3 minutes; Lane 2-4: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40728) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse bladder tissue with Mouse anti-Caveolin-1 antibody (<a href="/products/EM40728" style="font-weight: bold;text-decoration: underline;">EM40728</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM40728" style="font-weight: bold;text-decoration: underline;">EM40728</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue with Mouse anti-Caveolin-1 antibody (EM40728) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-Caveolin-1 antibody (<a href="/products/EM40728" style="font-weight: bold;text-decoration: underline;">EM40728</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM40728" style="font-weight: bold;text-decoration: underline;">EM40728</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-Caveolin-1 antibody (EM40728) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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