Skip to content

Cdk6 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# ER40101

Cdk6 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cdk6 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Cdk6 aa 267-326 / 326.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 37 kDa

Positive Control

HeLa cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, K562 cell lysate, COS-1 cell lysate, Hela, HepG2, human lung cancer tissue, mouse thymus tissue, rat thymus tissue.

Conjugation

unconjugated

RRID

AB_3069569

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:200

Target

Function

CDK6 protein contains a catalytic core composed of a serine/threonine domain.This protein also contains an ATP-binding pocket, inhibitory and activating phosphorylation sites, a PSTAIRE-like cyclin-binding domain and an activating T-loop motif. CDK6 is involved in a positive feedback loop that activates transcription factors through a reaction cascade. CDK6 is important for the control of G1 to S phase transition. However, in recent years, new evidence proved that the presence of CDK6 is not essential for proliferation in every cell type, the cell cycle has a complex circuitry of regulation and the role of CDK6 might be more important in certain cell types than in others, where CDK4 or CDK2 can act as protein kinases compensating its role.

Background References

1. "CDK6 blocks differentiation: coupling cell proliferation to the block to differentiation in leukemic cells." Matushansky I., Radparvar F., Skoultchi A.I. Oncogene 22:4143-4149(2003)

2. "Bone morphogenetic protein 2-induced osteoblast differentiation requires Smad-mediated down-regulation of Cdk6." Ogasawara T., Kawaguchi H., Jinno S., Hoshi K., Itaka K., Takato T., Nakamura K., Okayama H. Mol. Cell. Biol. 24:6560-6568(2004)

3. "CDK4 and CDK6 delay senescence by kinase-dependent and p16INK4a-independent mechanisms." Ruas M., Gregory F., Jones R., Poolman R., Starborg M., Rowe J., Brookes S., Peters G. Mol. Cell. Biol. 27:4273-4282(2007)

Sequence Similarity

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Tissue Specificity

Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.

Post-translational Modification

Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.

Subcellular Location

Cytoplasm, nucleus, Cell projection, Cytoskeleton.

UNIPROT

SwissProt: Q00534 Human

SwissProt: Q64261 Mouse

Entrez Gene: 114483 Rat

Synonyms

CDK 6 antibody

CDK6 antibody

CDK6_HUMAN antibody

Cell division protein kinase 6 antibody

Crk 2 antibody

Crk2 antibody

Cyclin dependent kinase 6 antibody

Cyclin-dependent kinase 6 antibody

MGC59692 antibody

p40 antibody

Expand

Images

  • Western blot analysis of Cdk6 on different lysates with Rabbit anti-Cdk6 antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: SK-OV-3 cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cdk6 on different lysates with Rabbit anti-Cdk6 antibody (ER40101) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: SK-OV-3 cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40101) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2,000 dilution.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: K562 cell lysate<br />Lane 2: COS-1 cell lysate

    Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2,000 dilution.
    Positive control:
    Lane 1: K562 cell lysate
    Lane 2: COS-1 cell lysate

  • ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining CDK6 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CDK6 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Cdk6 antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Cdk6 antibody (ER40101) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-Cdk6 antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-Cdk6 antibody (ER40101) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-Cdk6 antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER40101" style="font-weight: bold;text-decoration: underline;">ER40101</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-Cdk6 antibody (ER40101) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

metafield image

Cdk6 Recombinant Rabbit Monoclonal Antibody [SD20-50]

Application: IF-Cell,IF-Tissue,IHC-P,FC,WB

Reactivity: Human

Conjugate: unconjugated

Products with the same target and pathway

metafield image

Cdk6 Recombinant Rabbit Monoclonal Antibody [SD20-50]

Application: IF-Cell,IF-Tissue,IHC-P,FC,WB

Reactivity: Human

Conjugate: unconjugated

metafield image

Cdk6 Recombinant Rabbit Monoclonal Antibody [SD20-50] - BSA and Azide free

Application: IF-Cell,IF-Tissue,IHC-P,FC,WB

Reactivity: Human

Conjugate: unconjugated