Clathrin heavy chain Recombinant Rabbit Monoclonal Antibody [JA53-02]
Catalog# ET1704-50
Clathrin heavy chain Recombinant Rabbit Monoclonal Antibody [JA53-02]
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WB
-
IHC-P
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Human
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Mouse
-
Rat
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Green monkey
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unconjugated
Overview
Product Name
Clathrin heavy chain Recombinant Rabbit Monoclonal Antibody [JA53-02]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Clathrin heavy chain aa 201-250 / 1,675.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P
Molecular Weight
180 kDa
Positive Control
HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HUVEC cell lysate, SH-SY5Y cell lysate, COS-1 cell lysate, human colon cancer tissue.
Conjugation
unconjugated
Clone Number
JA53-02
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:200
Target
Function
Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are englufed by clathrin-coated vesicles. Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain binding site. The proximal segment of the Clathrin HC protein is essential for interactions between clathrin heavy chains and light chains which result in the formation of the triskelion structure.
Background References
1. McMichael, CM. et al. Mediation of clathrin-dependent trafficking during cytokinesis and cell expansion by Arabidopsis stomatal cytokinesis defective proteins. The Plant cell 25: 3910-25 (2013).
2. Van Damme, D. et al. Adaptin-like protein TPLATE and clathrin recruitment during plant somatic cytokinesis occurs via two distinct pathways. Proceedings of the National Academy of Sciences of the United States of America. 108: 615-20 (2011).
Sequence Similarity
Belongs to the clathrin heavy chain family.
Tissue Specificity
Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.
Subcellular Location
Cytoplasmic vesicle membrane.
Synonyms
CHC17 antibody
Clathrin heavy chain antibody
Clathrin heavy chain 1 antibody
Clathrin heavy chain 2 antibody
Clathrin heavy chain like 1 antibody
Clathrin heavy chain on chromosome 17 antibody
Clathrin heavy polypeptide antibody
CLH 17 antibody
CLH 22 antibody
CLH-17 antibody
ExpandImages
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Western blot analysis of Clathrin heavy chain on different lysates with Rabbit anti-Clathrin heavy chain antibody (ET1704-50) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: HUVEC cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 180kDa
Observed band size: 180 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Clathrin heavy chain antibody (ET1704-50) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-50) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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