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Cullin 3 Recombinant Rabbit Monoclonal Antibody [JE37-29]

Usd: 385

Specification

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Catalog# HA721711

Cullin 3 Recombinant Rabbit Monoclonal Antibody [JE37-29]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cullin 3 Recombinant Rabbit Monoclonal Antibody [JE37-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cullin 3 aa 719-768 / 768.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 89 kDa

Positive Control

SW480 cell lysate, SW620 cell lysate, HT-29 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human breast carcinoma tissue, human colon carcinoma tissue, human testis tissue.

Conjugation

unconjugated

Clone Number

JE37-29

RRID

AB_3072824

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Cullin 3 is a protein that in humans is encoded by the CUL3 gene. Cullin 3 protein belongs to the family of cullins which in mammals contains eight proteins (Cullin 1, Cullin 2, Cullin 3, Cullin 4A, Cullin 4B, Cullin 5, Cullin 7 and Cullin 9). Cullin proteins are an evolutionarily conserved family of proteins throughout yeast, plants and mammals. Cullin 3 is a component of Cullin-RING E3 ubiquitin ligases complexes (CRLs) which are involved in protein ubiquitylation and represent a part of ubiquitin–proteasome system (UPS). Added ubiquitin moieties to the lysine residue by CRLs then target the protein for the proteasomal degradation. Cullin-RING E3 ubiquitin ligases are involved in many cellular processes responsible for cell cycle regulation, stress response, protein trafficking, signal transduction, DNA replication, transcription, protein quality control, circadian clock and development.

Background References

1. Li X et al. CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy. 2021 Dec

2. Wu J et al. Cullin-3: Renal and Vascular Mechanisms Regulating Blood Pressure. Curr Hypertens Rep. 2020 Aug

Subcellular Location

Nucleus, Golgi apparatus, Cell projection, cilium, flagellum, Cytoplasm, cytoskeleton, spindle, cytoskeleton, microtubule organizing center, centrosome, cytoskeleton, spindle pole.

UNIPROT

SwissProt: Q13618 Human

SwissProt: Q9JLV5 Mouse

SwissProt: B5DF89 Rat

Synonyms

CUL 3 antibody

Cul-3 antibody

cul3 antibody

CUL3_HUMAN antibody

Cullin 3 antibody

Cullin-3 antibody

Cullin3 antibody

KIAA0617 antibody

PHA2E antibody

Images

  • Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate<br />Lane 2: SW620 cell lysate<br />Lane 3: HT-29 cell lysate<br />Lane 4: Mouse testis tissue lysate<br />Lane 5: Rat testis tissue lysate<br />Lane 6: Mouse brain tissue lysate<br />Lane 7: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 89 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (HA721711) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate
    Lane 2: SW620 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: Mouse testis tissue lysate
    Lane 5: Rat testis tissue lysate
    Lane 6: Mouse brain tissue lysate
    Lane 7: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 89 kDa
    Observed band size: 75 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721711) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cullin 3 antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cullin 3 antibody (HA721711) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721711) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cullin 3 antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cullin 3 antibody (HA721711) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721711) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Cullin 3 antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721711" style="font-weight: bold;text-decoration: underline;">HA721711</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Cullin 3 antibody (HA721711) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721711) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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