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Cyclin D1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0407-27

Cyclin D1 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cyclin D1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of cyclin D1.

Species Reactivity

Human, Mouse, Rat, Zebrafish

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 34 kDa

Positive Control

MCF-7 lysates, A549, HepG2, N2A, A431.

Conjugation

unconjugated

RRID

AB_3068641

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:200

  • FC

  • 1:500-1:1,000

Target

Function

Cyclin D1 is a protein that in humans is encoded by the CCND1 gene. The CCND1 gene encodes the cyclin D1 protein. The human CCND1 gene is located on the long arm of chromosome 11 (band 11q13). It is 13,388 base pairs long, and translates into 295 amino acids. Cyclin D1 is expressed in all adult human tissues with the exception of cells derived from bone marrow stem cell lines (both lymphoid and myeloid). The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDKs (Cyclin-dependent kinase). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Micrograph of cyclin D1 staining in a mantle cell lymphoma. Immunohistochemical staining of cyclin D1 antibodies is used to diagnose mantle cell lymphoma. Cyclin D1 has been found to be overexpressed in breast carcinoma. Its potential use as a biomarker was suggested.

Background References

1. LaBaer J et al. New functional activities for the p21 family of CDK inhibitors. Genes Dev 11:847-862 (1997).

2. Matsuura I et al. Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430:226-231 (2004).

Sequence Similarity

Belongs to the cyclin family. Cyclin D subfamily.

Post-translational Modification

Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.; Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation (By similarity). Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization.

Subcellular Location

Nucleus, Cytoplasm, Nucleus membrane.

UNIPROT

SwissProt: P24385 Human

SwissProt: P25322 Mouse

SwissProt: P39948 Rat

Synonyms

AI327039 antibody

B cell CLL/lymphoma 1 antibody

B cell leukemia 1 antibody

B cell lymphoma 1 protein antibody

B-cell lymphoma 1 protein antibody

BCL 1 antibody

BCL-1 antibody

BCL-1 oncogene antibody

BCL1 antibody

BCL1 oncogene antibody

Expand

Images

  • Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (<a href="/products/0407-27" style="font-weight: bold;text-decoration: underline;">0407-27</a>) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

    ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-27) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

  • ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (<a href="/products/0407-27" style="font-weight: bold;text-decoration: underline;">0407-27</a>) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

    ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-27) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

  • ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (<a href="/products/0407-27" style="font-weight: bold;text-decoration: underline;">0407-27</a>) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

    ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-27) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

  • Flow cytometric analysis of A431 cells labeling Cyclin D1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/0407-27" style="font-weight: bold;text-decoration: underline;">0407-27</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling Cyclin D1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (0407-27, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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