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Cytokeratin 17 Mouse Monoclonal Antibody [83-13]

Usd: 350

Specification

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Catalog# M0407-13

Cytokeratin 17 Mouse Monoclonal Antibody [83-13]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cytokeratin 17 Mouse Monoclonal Antibody [83-13]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Cytokeratin 17 aa 383-432 / 432.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 48 kDa

Positive Control

Hela cell lysate, Hela, PC-3M, SK-Br-3, human breast carcinoma tissue, mouse prostate tissue.

Conjugation

unconjugated

Clone Number

83-13

RRID

AB_3072978

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:100-1:200

Target

Function

Cytokeratin 17 may play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation

Background References

1. McGowan K.M et al. Keratin 17 expression in the hard epithelial context of the hair and nail, and its relevance for the pachyonychia congenita phenotype. J Invest Dermatol 114:1101-1107 (2000).

2. Shen Z et al. Altered keratin 17 peptide ligands inhibit in vitro proliferation of keratinocytes and T cells isolated from patients with psoriasis. J Am Acad Dermatol 54:992-1002 (2006).

Sequence Similarity

Belongs to the intermediate filament family.

Tissue Specificity

Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).

Post-translational Modification

Phosphorylation at Ser-44 occurs in a growth- and stress-dependent fashion in skin keratinocytes, it has no effect on filament organization.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q04695 Human

SwissProt: Q9QWL7 Mouse

SwissProt: Q6IFU8 Rat

Synonyms

39.1 antibody

CK 17 antibody

CK17 antibody

CK-17 antibody

Cytokeratin-17 antibody

K17 antibody

K1C17_HUMAN antibody

Keratin 17 antibody

keratin 17 epitope S1 antibody

keratin 17 epitope S2 antibody

Expand

Images

  • ICC staining Cytokeratin 17 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining Cytokeratin 17 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining Cytokeratin 17 in PC-3M cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining Cytokeratin 17 in PC-3M cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining Cytokeratin 17 in SK-Br-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining Cytokeratin 17 in SK-Br-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/M0407-13" style="font-weight: bold;text-decoration: underline;">M0407-13</a>) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M0407-13) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/M0407-13" style="font-weight: bold;text-decoration: underline;">M0407-13</a>) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M0407-13) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  • Western blot analysis of Cytokeratin 17 on different lysates with Mouse anti-Cytokeratin 17 antibody (<a href="/products/M0407-13" style="font-weight: bold;text-decoration: underline;">M0407-13</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa (Human cervical cancer cell) cell lysate<br />Lane 2: HEK-293 (Human embryonic Kidney cell) cell lysate<br />Lane 3: SiHa (Human cervical squamous cell) cell lysate<br />Lane 4: PC-3M (Human prostatic carcinoma cell) cell lysate<br /><br />Exposure time: 25 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/M0407-13" style="font-weight: bold;text-decoration: underline;">M0407-13</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa

    Western blot analysis of Cytokeratin 17 on different lysates with Mouse anti-Cytokeratin 17 antibody (M0407-13) at 1/1,000 dilution.

    Lane 1: HeLa (Human cervical cancer cell) cell lysate
    Lane 2: HEK-293 (Human embryonic Kidney cell) cell lysate
    Lane 3: SiHa (Human cervical squamous cell) cell lysate
    Lane 4: PC-3M (Human prostatic carcinoma cell) cell lysate

    Exposure time: 25 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: M0407-13, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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