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Cytokeratin 20 Mouse Monoclonal Antibody [A3H7]

Usd: 350

Specification

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Catalog# EM1901-97

Cytokeratin 20 Mouse Monoclonal Antibody [A3H7]

Application

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cytokeratin 20 Mouse Monoclonal Antibody [A3H7]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal Human Cytokeratin 20.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 48 kDa

Positive Control

Human colon carcinoma tissue, human small intestine tissue, human colon tissue.

Conjugation

unconjugated

Clone Number

A3H7

RRID

AB_3068942

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • IHC-P

  • 1:800-1,000

  • IF-Tissue

  • 1:500

Target

Function

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to CK20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the CK20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumour.

Background References

1. You X. et. al. Aberrant Cytokeratin 20 mRNA Expression in Peripheral Blood and Lymph Nodes Indicates Micrometastasis and Poor Prognosis in Patients With Gastric Carcinoma. Technol Cancer Res Treat. 2019 Jan.

2. Al-Maghrabi J. et. al. Immunohistochemical staining of cytokeratin 20 and cytokeratin 7 in colorectal carcinomas: Four different immunostaining profiles. Saudi J Gastroenterol. 2018 Mar-Apr.

Sequence Similarity

Belongs to the intermediate filament family.

Tissue Specificity

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

Post-translational Modification

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).; Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P35900 Human

Synonyms

CK 20 antibody

CK-20 antibody

CK20 antibody

Cytokeratin-20 antibody

Cytokeratin20 antibody

K1C20_HUMAN antibody

K20 antibody

KA20 antibody

Keratin 20 antibody

keratin 20, type I antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-Cytokeratin 20 antibody (<a href="/products/EM1901-97" style="font-weight: bold;text-decoration: underline;">EM1901-97</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-97" style="font-weight: bold;text-decoration: underline;">EM1901-97</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-Cytokeratin 20 antibody (EM1901-97) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-97) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Mouse anti-Cytokeratin 20 antibody (<a href="/products/EM1901-97" style="font-weight: bold;text-decoration: underline;">EM1901-97</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-97" style="font-weight: bold;text-decoration: underline;">EM1901-97</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Mouse anti-Cytokeratin 20 antibody (EM1901-97) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Cytokeratin 20 antibody (<a href="/products/EM1901-97" style="font-weight: bold;text-decoration: underline;">EM1901-97</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-97" style="font-weight: bold;text-decoration: underline;">EM1901-97</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Cytokeratin 20 antibody (EM1901-97) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: small intestine<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: small intestine

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Appendix<br />Sample: Paraffin-embedded section<br /><br />Primary antibody dilution: 1/400<br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes<br />Platform: Leica Biosystems BOND&reg; RX

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Appendix
    Sample: Paraffin-embedded section

    Primary antibody dilution: 1/400
    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes
    Platform: Leica Biosystems BOND® RX

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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